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Citations for GE Life Sciences :
1 - 50 of 2723 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the 5’ half of the tRNA (nucleotides 1-39) was chemically synthesized (GE Healthcare Dharmacon) and enzymatically ligated to the chemically synthesized 3’ half following established protocols (52 ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated biotin and aggregated viruses were removed by NAP-5 gel filtration columns (GE Healthcare) and 0.2 μm pore size filters ...
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bioRxiv - Microbiology 2020Quote: ... Biotin CAPture Reagent provided in the Biotin CAPture Kit (GE Healthcare) was captured onto the sensor chip according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... Individual tRNA species were bound to biotinylated DNA probes anchored to high-capacity streptavidin agarose resin (GE Healthcare) in 30 mM Hepes-KOG ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Biochemistry 2022Quote: ... Individual tRNA species were purified using biotinylated DNA probes anchored to a high-capacity streptavidin agarose resin (GE Healthcare). Typically for this purification ...
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bioRxiv - Cell Biology 2019Quote: ... 6His-Cdc11-SNAP was biotinylated with BG-Biotin and subsequently immobilized on a CAP chip (Biotin Capture Kit; GE Healthcare, Freiburg, Germany). Preparation of the chip ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µg of a 5’ amino-modified DNA oligonucleotide complementary to the target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 mL, GE Healthcare). For isolation of full-length tRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 µg of a 5' amino-modified DNA oligonucleotide (Table S1) complementary to target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 ml, GE Healthcare). Binding to the NHS column was performed by recirculation of the small RNA fraction over the complementary NHS columun for 3 hours at 65°C ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies were then purified using size exclusion columns (Illustra NAP-5 columns, #17085302, GE Healthcare). The absorption of IgG at 280 nm and Cy3B dye at 559 nm of the reaction products was measured by spectrophotometry (NanoDrop ND-1000 Spectrophotometer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... Biotin excess was removed by SEC on a Superdex 200 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Biophysics 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... followed by the removal of biotin by desalting on PD10 columns (GE Healthcare). The desalted lysates were then applied to streptavidin agarose resin (100 µl ...
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bioRxiv - Immunology 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare). The control MHC-I (HLA-A2 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Immunology 2020Quote: ... Biotin-labeled SUMO-fusion TRIM14 was coupled on the sensor chip SA (GE Healthcare). Dilution series of TBK1 or IRF-3 (1.25 ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare) for the RBD probe or a HiLoad Superose 6 16/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and unincorporated biotin-17-dCTP was removed by using S-200 HR Microspin columns (GE Healthcare). The DNA was ethanol precipitated and resuspended in TE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
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bioRxiv - Biochemistry 2021Quote: ... Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v, GE Healthcare), and vimentin levels estimated by western blot with anti-vimentin V9 monoclonal antibody (1:1000 v/v ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labelled HCV 3’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Unreacted biotin label was removed via SEC on a Superdex 200 Increase column (GE Healthcare Life Sciences). Biotinylated BSA-Lewis X was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
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bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Systems Biology 2020Quote: ... The signal of biotin-bound HRP was visualized using Amersham ECL Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Excess biotin was removed by size-exclusion chromatography on a S200 10/300 GL column (GE Healthcare) with a distal 1 ml GSTrap 4B (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Biotinylation was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...