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Citations for GE Life Sciences :
1 - 50 of 688 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat AntiRat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1 hr at 24 °C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were loaded into a ResourceS (GE Healthcare) cartridge and eluted in a gradient of 0 to 500 mM NaCl in 50 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with lgG sepharose (GE Healthcare) at 4 °C for 2 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with glutathione sepharose beads (GE Healthcare) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysates were incubated with IgG-Sepharose (GE Healthcare) at 4 °C for 2 h and eluted by TEV protease (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... lysates were incubated with streptavidin sepharose beads (GE Healthcare) overnight while rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
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bioRxiv - Biophysics 2019Quote: ... Lysate was loaded on to HisTrap HP (GE Healthcare), His-RanQ69L was eluted in binding buffer with 500mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were loaded onto HisTrap crude column (GE Healthcare) on FPLC system (AKTA pure) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysates were incubated with IgG Sepharose (GE Healthcare), washed with buffer Y and eluted by overnight TEV cleavage.
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bioRxiv - Cell Biology 2023Quote: ... lysates were incubated with glutathione Sepharose beads (GE Healthcare) coated with the other interaction partner for 30min at 4°C on a rotary wheel ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Bioengineering 2020Quote: ... Clarified lysate loaded onto HiTrap Q HP column (GE Healthcare) and eluted through salt gradient using low-salt buffer (as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant proteins were eluted by imidazole gradient ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare), eluted with denaturing buffer supplemented with 500 mM imidazole and refolded as indicated in Table S3 using MWCO 2000 membranes (Spectra/Por) ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was purified by Histrap FF columns (GE Healthcare) with an imidazole gradient from 0 to 0.5 M ...
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bioRxiv - Cell Biology 2020Quote: ... Centrifuge-cleared lysate was applied to Ni sepharose (GE healthcare), washed with 50 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... protein lysates were incubated with glutathione Sepharose beads (GE Healthcare) in E buffer for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were incubated with prewashed glutathione sepharose (GE Healthcare) and gently mixed with rotation for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with protein G sepharose beads (GE Healthcare) bound to α-PP2A-Aα or control IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... the cleared lysate was applied to Streptactin beads (GE Healthcare) in batch and incubated for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with IgG sepharose 6 beads (GE Healthcare) in XB buffer (100 mM KCl ...
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bioRxiv - Biophysics 2019Quote: ... Cleared lysate was loaded onto a glutathione-sepharose (GE Healthcare) column (GST-column ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were incubated with glutathione Sepharose 4B (GE Healthcare), washed with wash buffer (20 mM Tris pH 8.5 [pUL21-GST] or 7.5 [GST-PP1γ] ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was incubated with glutathione-Sepharose resin (GE Healthcare) for several hours before washing the resin first with PBS buffer containing 1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were mixed with either StrepTactin sepharose (GE Healthcare) for PorEStrep immunoprecipation ...
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bioRxiv - Microbiology 2019Quote: ... Cleared lysates were then incubated with glutathione sepharose (GE healthcare) for 4 hours at 4°C and ...
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bioRxiv - Microbiology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant protein was eluted by imidazole gradient ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with pre-equilibrated streptavidin beads (GE Healthcare) for 4 hr ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with glutathione sepharose (GE Healthcare) and then washed with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare) and eluted with denaturing buffer or non-denaturing buffer supplemented with 500 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were precleared with Gelatin Sepharose 4B beads (GE Healthcare) for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was loaded on HisTrap HP Column (GE Healthcare) and eluted on the AKTA FPLC ...
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bioRxiv - Cell Biology 2024Quote: ... the clarified lysate was loaded onto glutathione sepharose (GE healthcare) and eluted using elution buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared lysates were loaded onto GST-Trap (GE Healthcare Life Sciences) washed with 20-30 column volumes wash buffer (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was incubated with Gluthatione Sepharose™ 4B (GE Healthcare) for 2 h and then washed in wash buffer containing 200 mM Tris (pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were passed on preequilibrated Histrap HP columns (GE Healthcare). The columns were washed with wash buffer (lysis buffer with 40 mM Imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were incubated with magnetic beads (Dynal M260; GE Healthcare) coated with anti-Flag antibody (M2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Clarified lysate was applied to a Glutathione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was applied to Ni2+-NTA resin (GE Healthcare), washed with Ni-buffer C (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was loaded onto a HisTrap column (GE Healthcare) and eluted with low salt ...
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bioRxiv - Molecular Biology 2019Quote: ... protein lysate samples were transferred to a nitrocellulose membrane (GE Healthcare). The membrane was blocked in 5% milk in TBST ...
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bioRxiv - Biochemistry 2019Quote: ... lysate was loaded onto Heparin column (HiTrap Heparin column, GE Healthcare). RNAP was further purified using size exclusion (Superose 6 ...