Labshake search
Citations for GE Life Sciences :
1 - 50 of 63 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Digested peptides were separated from the solution and retained by a solid phase extraction system (SepPak, Waters) and subsequently enriched by SEC on an ÄKTAmicro chromatography system (GE Healthcare) using a Superdex™ Peptide 3.2/300 column (GE Healthcare ...
-
bioRxiv - Microbiology 2020Quote: Solid-phase reversible immobilisation (SPRI) on carboxylated paramagnetic beads (Sera-Mag™ Magnetic SpeedBeads™, from GE Healthcare) were prepared for RNA binding as described (https://openwetware.org/wiki/SPRI_bead_mix) ...
-
bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
-
bioRxiv - Genomics 2021Quote: ... and DNA was bead purified with 1.6x homebrew solid phase reversible immobilization (SPRI) beads (1mL Sera-Mag SpeedBeads (GE Healthcare), 9g PEG 8000 (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... reverse-crosslinked samples were combined and the DNA was purified using solid phase reverse immobilization (SPRI) Sera-Mag SpeedBeads (1.6:1 ratio, GE Healthcare). The final library was eluted with 22 μL ddH2O.
-
bioRxiv - Biochemistry 2020Quote: ... and Single-Pot Solid-Phase enhanced Sample Preparation (SP3) using paramagnetic beads (GEHE45152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophilic, GE Healthcare) and GEHE65152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic ...
-
bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was cleaned up using solid phase reversible immobilization (SPRI) beads (Sera Mag™, GE Healthcare Life Sciences, Chicago, IL, USA) in a homemade bead binding buffer containing 22% polyethylene glycol (PEG) ...
-
bioRxiv - Genomics 2023Quote: ... The phage DNA is then purified using 1.6x volume of homebrewed solid phase reversible immobilization (SPRI beads) (1mL Sera-Mag SpeedBeads (GE Healthcare, GE1715210401150), 9g PEG 8000 (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Reduced lysate was further processed with single-pot solid-phase-enhanced (SP3) bead technique45 using hydrophilic and hydrophobic Sera-Mag Speed Beads (GE Life Sciences, Issaquah, Washington, United States). Proteins were bound to paramagnetic beads with 100% ethanol (80% v/v) ...
-
bioRxiv - Cell Biology 2021Quote: ... and an Insight solid-state illumination unit (Applied Precision Ltd.). Images shown as Z-stacks in Figure 2-figure supplement 2A ...
-
bioRxiv - Cell Biology 2020Quote: ... and an Insight solid-state illumination unit (Applied Precision Ltd.). Images shown as Z stacks were acquired and processed by iterative deconvolution using SoftWoRx software (Applied Precision Ltd.) ...
-
bioRxiv - Microbiology 2021Quote: ... with a sCMOS (PCO-edge) camera and a DV Trulight solid state illumination module (GE Healthcare), and a 100x/1.40 oil-immersion objective ...
-
bioRxiv - Genetics 2023Quote: ... with a sCMOS (PCO-edge) camera and a DV Trulight solid-state illumination module (GE Healthcare), and a ×100/1.40 oil-immersion objective ...
-
The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... the aqueous phase filtered through a G25 MicroSpin column (GE healthcare) and the flowthrough precipitated in 75% isopropanol with 40µg of glycogen overnight at −20°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... A PhytoPure DNA Extraction kit (GE Healthcare) was used to complete the DNA extraction ...
-
bioRxiv - Microbiology 2019Quote: ... Phase-contrast images were acquired on a DV Elite microscope (GE healthcare) equipped with a sCMOS (PCO ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Reverse phase FPLC on a Resource RPC column (GE Healthcare, Danderyd, Sweden) in acetonitrile gradient in 0.1% trifluoroacetic acid was used for final purification of the mature peptides ...
-
bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
-
bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
-
bioRxiv - Bioengineering 2019Quote: ... Valve strips were prepared by printing 1.25 mm wide lines of solid wax-ink (Black ColorQube ink, Xerox, Norwalk, CT) onto cellulose membranes (Chr1, GE Healthcare, Pittsburgh, PA) using a Xerox ColorQube 8570 (Norwalk ...
-
bioRxiv - Biophysics 2020Quote: ... the protein was separated on a RESOURCE™ reversed phase chromatography column (GE Healthcare) using a 0-70 % acetonitrile gradient ...
-
bioRxiv - Microbiology 2022Quote: ... or lysed for RNA extraction (GE Healthcare, Buckinghamshire, UK). LT and mutant LT (E211K ...
-
bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
-
bioRxiv - Microbiology 2019Quote: ... using ‘Illustra DNA extraction kit Phytopure’ (GE Healthcare, Little Chalfont, UK). To ensure that DNA originated from endophytic microorganisms ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and resuspended them in Protein Extraction Buffer-V (GE Healthcare, Uppsala, Sweden) (urea ...
-
bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted using the QuickPrep RNA extraction kit (GE HealthCare). Reverse transcribed using the TaqMan Reverse Transcription Reagent (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2021Quote: ... Residual NaCO3 from the reverse phase were removed using a PD-10 desalting column (GE Healthcare Life Sciences). Before aggregation ...
-
bioRxiv - Neuroscience 2022Quote: RNA extraction was performed using illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... The water phase was loaded to a MonoQ 5/50 GL (1 ml) column (GE17-5166-01, GE Healthcare). ppGpp was eluted with a linear gradient from 0.5 mM to 600 mM LiCl in buffer C (25 mM Tris-HCl pH 8.3 ...
-
bioRxiv - Microbiology 2020Quote: ... then 1.5 column volume of mobile phase (DPBS) was run through the column at flow rate of 0.5 mL/min using the AKTA Pure (GE Healthcare) system ...
-
bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
-
bioRxiv - Microbiology 2022Quote: RNA was extracted using Illustra RNAspin Mini RNA extraction kit (GE Healthcare, Buckinghamshire, UK). Three biological replicates per treatment (untreated and LT treated ...
-
bioRxiv - Neuroscience 2019Quote: ... peptides were further fractionated prior to LC-MS by reversed-phase HPLC using an Ettan ÄKTA micro HPLC system (GE Healthcare), as described elsewhere98.
-
bioRxiv - Cell Biology 2019Quote: ... G2 and G1 phases based on the signal intensity of anti-CENPF antibody staining with software (IN Cell Investigator, GE Healthcare).
-
bioRxiv - Microbiology 2019Quote: ... Pnf was purified over HisTrap™ Ni2+-affinity column with the fast phase liquid chromatography (FPLC) AKTA system as per the manufacturer’s protocol (GE Healthcare). Plasmids pGEX-2T-wtRhoA ...
-
bioRxiv - Biophysics 2022Quote: ... The flow through was collected and used for further purification by reversed-phase chromatography using a Resource RPC column (GE Healthcare), equilibrated in MQ with 0.08% TFA (v/v),and eluted with a linear gradient from 0-100% of 70% acetonitrile (v/v) ...
-
bioRxiv - Cell Biology 2022Quote: ... A total of 15 raw images (five phases, three rotations) per plane were collected and reconstructed by using SoftWoRx software (Applied Precision), processed in ImageJ/Fiji (Schindelin et al. ...
-
bioRxiv - Microbiology 2023Quote: ... The quantitative analysis of lipids extracted to the butanol phase was carried out by phosphorimaging in a StormTM imaging system (GE Healthcare) or PMA staining and analysis performed using Image Quant TL ...
-
bioRxiv - Biochemistry 2023Quote: ... The clear top phase obtained above a white band was collected and separated over a 5 ml HiTrap Q FF column (GE Healthcare) equilibrated with buffer A ...
-
bioRxiv - Molecular Biology 2024Quote: ... Phase contrast and epifluorescence images to detect mTurquoise2 fluorescence were acquired on a DeltaVision Elite V3 inverted fluorescence microscope (GE Healthcare) with a CFP filter set (excitation = 400-453 nm ...
-
bioRxiv - Microbiology 2021Quote: ... and total RNA extraction was performed using Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). P ...
-
bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the aqueous phase was used for size-exclusion chromatography by using a disposable PD SpinTrap G-25 column (GE Healthcare Life Sciences). The purified tRNALeu was then ethanol precipitated ...
-
bioRxiv - Cell Biology 2021Quote: Cells were grown in appropriate synthetic dropout media at 30°C to mid-log phase (OD600 of ∼0.5) and imaged using DeltaVision Elite system (GE Healthcare Life Sciences) equipped with an Olympus IX-71 inverted microscope ...
-
bioRxiv - Biochemistry 2020Quote: ... DNA was purified by phenol:chloroform extraction and centrifugation through a G-25 spin column (GE Healthcare). Samples were analyzed by agarose gel-electrophoresis and post-run ethidium-bromide stain.
-
bioRxiv - Biochemistry 2020Quote: ... DNA was isolated by phenol:chloroform extraction and centrifugation through a G-25 spin column (GE Healthcare). Samples were fractionated on 0.8 % denaturing agarose gel in 30 mM NaOH / 2 mM EDTA ...
-
bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted using gSYNC DNA Extraction kit (Geneaid) and quantified by nanovue (GE Healthcare). A touchdown PCR was performed on 200 ng of extracted genomic DNA with primers P488 and P650 to amplify a 759 bp fragment of ATP7B containing exon 6 and surrounding intronic regions ...
-
bioRxiv - Biochemistry 2020Quote: ... The clear upper phase of the lysate was desalted by size exclusion chromatography using a PD MiniTrap G-25 prepacked column (GE Healthcare, Chicago, IL, USA) with assay buffer as eluent ...