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Citations for GE Life Sciences :
1 - 50 of 4559 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Fully formed complexes and disassembled subunits were separated on a Superose 6 10/300 column (GE Healthcare) equilibrated in SEC buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Microbiology 2021Quote: ... Plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Immunology 2020Quote: ... Plates were scanned on the Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare). Data was analyzed using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed thrice in PBS and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 µm; GE Healthcare). All staining steps were performed at room temperature for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare). Entry routes were determined by pre-treating monolayers of VeroE6 or VeroE6-TMPRSS2 cells with a concentration range of camostat mesylate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 mm; GE Healthcare). All pseudovirus experiments were quantified using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Alpha-synuclein was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction column (GE Healthcare) (76) ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was quantified using the Alpha Innotech software for the FluoroChemQ chemiluminescent exposure system (Alpha Innotech; GE Healthcare, London, ON, Canada) or ImageLab for ChemiDoc system (BioRad ...
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bioRxiv - Genetics 2024Quote: ... without Alpha-Thioglycerol (GE Healthcare Life Sciences Cat. # SH30228.01); 1X GlutaMAX-I ...
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bioRxiv - Molecular Biology 2022Quote: ... and the σA subunit were expressed and purified by chromatography on HisTrap HP (GE Healthcare) Ni2+-affinity columns as described (Hu et al. ...
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bioRxiv - Systems Biology 2022Quote: ... and Cy5 channels on either a cellWoRx automated imaging platform (Applied Precision) or in the DAPI and Cy5 channels of a Cellomics ArrayScan VTi (Thermo Fisher).
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bioRxiv - Bioengineering 2023Quote: ... with an 8-channel cardiac phased-array coil (GE Healthcare, Waukesha, WI). Volunteers performed tidal breathing in a supine position ...
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bioRxiv - Cell Biology 2022Quote: ... SIM reconstruction and registration of channels were performed by softWoRX software (GE Healthcare). For SR-SIM Elyra system ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescently labelled subunits were assessed by visualizing an SDS-PAGE gel with Typhoon FLA 9500 (GE Healthcare) with LPG filter and 100 μm pixel size ...
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bioRxiv - Neuroscience 2022Quote: ... equipped with an 8-channel receive-only head array coil (GE Healthcare, Milwaukee, WI). First ...
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bioRxiv - Neuroscience 2022Quote: ... along with channel-specific optical transfer functions in the softWoRx software package (Applied Precision) and the maximum projection of several Z-planes obtained with ImageJ software ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Biochemistry 2020Quote: ... Detection of radiolabelled mt-DNA subunits was performed using an Amersham™ Typhoon™ Biomolecular Imager (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... using a CT-scanner (CT; 16-channel multidetector; BrightSpeed Elite, GE Healthcare, Fairfield, CT, USA) for additional confirmation.
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bioRxiv - Cell Biology 2019Quote: ... Raw OMX data were reconstructed and channel registered in SoftWoRx software version 6.1.3 (Applied Precision).
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA was visualized using a FAM channel on a Typhoon FLA 7000 instrument (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The gels were scanned using a FAM channel on a Typhoon FLA 7000 instrument (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were scanned on the Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... filter setting of between 0.001 and 0.003 and channel-specific OTFs employing the softWoRx version 6.5.2 software package (GE Healthcare) to obtain a super-resolution image stack ...
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Immunology 2019Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Cell Biology 2020Quote: ... Lateral color channel alignment was performed using a special image registration slide and algorithm provided by GE Healthcare. Correct 3D alignment was confirmed and refined in z by a custom test sample with two layers of 0.2 μm diameter TetraSpeck beads (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) and SoftWoRx ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed and scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 25 μm; GE Healthcare). All staining steps were performed at room temperature for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (10600023; GE Healthcare Life Sciences, Chalfont, UK). After blocking with 5% skim milk (232100 ...
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bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...