Labshake search
Citations for GE Life Sciences :
1 - 49 of 49 citations for Sar1 Angiotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Sar1 was affinity purified following application to a 5 mL HisTrap column (GE Healthcare) equilibrated in lysis/binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... A Multiphor II (GE Healthcare) was used for isoelectric focusing ...
-
bioRxiv - Physiology 2020Quote: ... and a Multiphor II Electrophoresis System (GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
-
bioRxiv - Biochemistry 2023Quote: ... Streptactin II agarose was from GE healthcare (Little Chalfont, UK). Myc-trap agarose was from Chromotek (Planegg ...
-
bioRxiv - Microbiology 2021Quote: ... The probe was produced with a Rediprime II labeling kit (GE Healthcare). Hybridization was performed as described by Dienst et al ...
-
bioRxiv - Microbiology 2021Quote: ... Images were captured by Photometrics Cascade II EMCCD camera and processed by SoftWoRx imaging software (Applied Precision). For counting hooks ...
-
bioRxiv - Microbiology 2019Quote: ... Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare) in three running phase (phase 1 ...
-
bioRxiv - Bioengineering 2023Quote: ... and supplemented with 5 % Cytiva HyClone Fetal Clone II (GE Healthcare Lifesciences), MITO+ (1:1,000 dilution ...
-
bioRxiv - Cancer Biology 2019Quote: ... and labelled using a standard Rediprime II Random Prime labelling system (GE healthcare) and radioactive [α-32P] dCTP ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... (Manassas, VA) and Cytiva HyClone Fetal Clone II was purchased from GE Healthcare Life Sciences.
-
bioRxiv - Cell Biology 2023Quote: ... Pol II was bound to protein G Sepharose Fast Flow beads (GE Healthcare) containing 8WG16 antibody against Rpb1 ...
-
bioRxiv - Microbiology 2020Quote: ... 1D gel electrophoresis analysis was performed with Ettan IPGphor II control software (GE Healthcare). For 2D gel electrophoresis ...
-
bioRxiv - Microbiology 2019Quote: ... supplemented with 10% serum (HyClone FetalClone II, GE Healthcare or Fetal Bovine Serum, Invitrogen), 100 U/mL penicillin and streptomycin (Gibco) ...
-
bioRxiv - Bioengineering 2020Quote: ... containing 10% Heat Inactivated HyClone™ FetalClone™ II Serum (U.S.) (GE Healthcare Life Sciences: SH30066.03) and 1% penicillin-streptomycin (GIBCO ...
-
bioRxiv - Bioengineering 2020Quote: ... containing 10% Heat Inactivated HyClone™ FetalClone™ II Serum (U.S.) (GE Healthcare Life Sciences: SH30066.03) and 1% penicillin-streptomycin (GIBCO ...
-
bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... Individual images were also obtained with a 100x objective on a DeltaVision II deconvolution microscope (Applied Precision Inc. ...
-
bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
-
bioRxiv - Molecular Biology 2019Quote: ... was labeled with dCTP α-32P using Amersham Rediprime II DNA Labelling kit (GE Healthcare, Buckinghamshire, England) and used for hybridization at 42 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... IEF was then performed on a Multiphor II Electrophoresis System with ImmobilineDryStrip Kit (Amersham Biosciences, Ge Healthcare), in 4 consecutive steps ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Amersham Rediprime II Random Prime Labeling System (RPN 1633; GE Healthcare, Chalfont St Giles, UK). Membranes were hybridized (250 mM Na2HPO4 ...
-
bioRxiv - Biochemistry 2023Quote: MALS experiments were done on a Wyatt HELEOS II scattering detector coupled to an ÄKTA FPLC (GE Healthcare) with a Superose 6 Increase 10/300 column using HBS as the sample and running buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... The assembled transcribing Pol II complex was then applied onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated with the SEC150 buffer (20 mM HEPES pH 7.4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The general purification strategy was as follows: (i) Nickel (II) affinity chromatography (Ni Sepharose 6 Fast Flow, GE Healthcare) in 25 mM Tris (pH 8.0) ...
-
bioRxiv - Genomics 2022Quote: ... Dual X-ray absorptiometry (DXA) was performed on the femurs and lumbar vertebrae using the Lunar Piximus II (GE Healthcare) as described previously by Beamer et al57 ...
-
bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
-
bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
-
bioRxiv - Microbiology 2022Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
-
bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...
-
bioRxiv - Microbiology 2022Quote: ... and the obtained supernatant was purified using 0.22 μm filter and passed over a Strep-Tactin II column (GE Healthcare) to collect secreted TraC ...
-
bioRxiv - Microbiology 2023Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
-
bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... The gel was stained in Sybr Green II and scanned on a Typhoon 9410 Variable Mode Imager (GE Healthcare, Piscataway, NJ).
-
bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
-
bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...
-
bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... size exclusion chromatography (SEC) coupled to MALS analysis was performed on MiniDAWN TREOS II coupled to S200 10/300 increase column (GE Healthcare) mounted on FPLC system (AKTA purifier) ...
-
bioRxiv - Cell Biology 2023Quote: SEC-MALS experiments were carried out on DAWN HELEOS-II (Wyatt Technology Corporation) coupled with a Shimadzu liquid chromatography system equipped with a Superdex 200 10/300 GL (GE Healthcare) gel-filtration column ...
-
bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
-
bioRxiv - Bioengineering 2020Quote: ... MRI scan acquired using a Dixon imaging sequence (Table II) acquired between two and four weeks before surgery on a clinical 3T MRI (Discovery MR750, GE Healthcare, Waukesha, WI). Regions of interest (ROIs ...
-
bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Probes were labled with 32P-dCTP (Hartmann Analytic, Braunschweig) by the use of the Rediprime II DNA Labeling Kit (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual ...
-
bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto a nickel (II) 5 mL HisTrap packed with Sepharose® 6 fast flow agarose beads (GE Healthcare Biosciences, NSW, Australia) using an ÄKTApure™ system (GE Healthcare Biosciences) ...
-
bioRxiv - Genetics 2021Quote: ... Twenty-five ng were labelled with 50 µCi [α- 32P]dCTP (SRP-305; Hartmann Analytic; Braunschweig; Germany) with the Amersham Rediprime II Random Prime Labeling System (RPN1633; GE Healthcare, Chalfont St Giles, UK) and purified on Illustra G-50 ProbeQuant micro columns (GE Healthcare ...