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Citations for GE Life Sciences :
1 - 50 of 4947 citations for Retinol Binding Protein High Sensitivity ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... Marker proteins were from the isoelectric focusing calibration kit (high pH range, GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Biochemistry 2024Quote: ... and detected using a high-sensitivity imaging system (LAS-4000, GE healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Biochemistry 2021Quote: ... All columns were calibrated using protein standards from both high and low molecular weight kits (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Sample protein masses were assessed with Amersham high molecular weight protein marker mix (GE Healthcare) with proteins porcine thyroglobulin (Rs=17.0nm) ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Cell Biology 2019Quote: ... The column was calibrated with protein standards of known molecular weight and stokes’ radius (Low and High Molecular Weight Kit, GE Healthcare) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Biochemistry 2024Quote: ... calibrated with High Molecular Weight calibration kit (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was affinity purified with streptavidin sepharose high performance beads (GE Healthcare), washed three times with l × lysis buffer containing 200 mM NaCl and 0.1% TritonX-100 ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was purified on a Ni Sepharose High Performance column (GE Healthcare). The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Bioengineering 2024Quote: ... SpyTag003-linked non-antigen binding proteins) were injected onto a pre-equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare), or a HiLoad 16/600 Superdex 75 pg column (SUMO-SpyTag003 and affibodies ...
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bioRxiv - Microbiology 2022Quote: ... IgG was isolated using 5 ml HiTrap Protein G High Performance column (GE Healthcare), whereas IgM was isolated using POROS™ CaptureSelect™ IgM Affinity Matrix (ThermoScientific ...
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bioRxiv - Biophysics 2023Quote: ... Extracted protein lysates were mixed with Streptavidin Sepharose High-Performance beads (GE Healthcare, USA) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... A gel filtration calibration kit (high molecular weight; GE Healthcare) with protein standards including thyroglobulin (669 kDa) ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein from each sample were added to 50 µl of precleaned CaM binding beads (GE Healthcare, 17-0529-01) in 300 to 500 µl of 1% TX100-TNM lysis buffer and incubated with rotation at 4°C overnight (16-18 hours) ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Systems Biology 2021Quote: ... SIL-proteins were purified using the Ni-Sepharose High-Performance resin (GE Healthcare Life Sciences) and precipitated using methanol:chloroform:water precipitation in Eppendorf Protein LoBind® tubes ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...