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Citations for GE Life Sciences :
1 - 50 of 1642 citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Plant Biology 2019Quote: ... GST-tagged and His-tagged proteins were affinity-purified using glutathione agarose beads (GE Healthcare, Pittsburgh, PA, USA) and Ni-NTA affinity agarose beads (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... Purifications of the GST-tagged recombinants were performed as described by the manufacturer (GE Healthcare). For expression in mammalian cells ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Plant Biology 2019Quote: ... Glutathione S transferase (GST)-tagged recombinant proteins were purified using glutathione sepharose 4B (GE Healthcare, #17-0756-01) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was purified by TALON Superflow (GE Healthcare Life Sciences, Pittsburgh, PA, USA) according to the manufacturer’s description ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Biophysics 2020Quote: ... The GST was cleaved with GST-tagged HRV 3C protease (PreScission Protease, GE Healthcare). The GST and protease was removed with glutathione sepharose resin ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-tagged or GST-tagged proteins immobilized on Amylose beads (GE Healthcare Life Science) or Glutathione Sepharose (GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged expressed proteins were purified using GST beads (Glutathione Sepharose 4B; GE Healthcare Life Science). On the following day ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tagged TMIGD1 constructs in pGEX-4T-1 (GE Healthcare): GST-TMIGD1 (hTMIGD1 cytoplasmic tail (AA 242-262) ...
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bioRxiv - Developmental Biology 2019Quote: GST-tagged protein and bounded by Glutathione beads (GE healthcare). 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3) ...
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... vector and GST-tagged or MBP-tagged proteins were captured using Glutathione Sepharose beads (GE Healthcare) or amylose resin (New England Biolabs) ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Genetics 2019Quote: ... All GST-tagged proteins were purified using glutathione beads (GE Healthcare) according to the protocol supplied by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... The GST-tagged constructs were purified with glutathione beads (GE Healthcare), whereas ion metal affinity chromatography using Ni2+ beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-tagged protein was purified by Glutathione sepharose (GE Healthcare), followed by on-column TEV cleavage ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Physiology 2019Quote: ... GST-tagged protein from supernatant were purified using glutathione-Sepharose 4B (GE Healthcare). Sepharose bound proteins were used as the protein columns ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Microbiology 2020Quote: His-tagged HexaPro was immobilized to a NiNTA sensorchip (GE Healthcare) to a level of ∼500 response units (RUs ...
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bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biophysics 2021Quote: ... The GST tagged Gbp2 proteins were purified using glutathione sepharose 4B resin (GE Healthcare) followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: GST-tagged proteins were purified using affinity chromatography with Glutathione Sepharose beads (GE Healthcare) in a cold room ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tagged proteins were absorbed with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5hrs and recovered by 3C protease cleavage at 4°C overnight in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged proteins were purified with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01), washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare ...