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Citations for GE Life Sciences :
1 - 50 of 3592 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Immunology 2023Quote: ... CNBr-activated Sepharose beads (GE Healthcare) were used for coupling of the I-A/I-E-specific antibody (M5/114.15.2 ...
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bioRxiv - Cell Biology 2023Quote: ... CNBr activated Sepharose 4B (GE Healthcare) were let to swell in 1 mM HCl pH 3.0 for 30 min and washed ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Activated Thiol Sepharose 4B (GE Healthcare Life Sciences) was added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
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bioRxiv - Biochemistry 2020Quote: ... onto an activated PDVF membrane (GE Healthcare Life Sciences) followed by blocking with TBST/5% wt/vol non-fat milk (1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to activated PVDF membranes (GE Healthcare) using the semi-dry method for 1h at 24 V ...
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bioRxiv - Biochemistry 2022Quote: ... to cyanogen bromide (CNBr)-activated Sepharose 4B (GE Healthcare) according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2022Quote: ... coupled to N-hydroxysuccinimidyl (NHS)-activated Sepharose (GE Healthcare) was used to immunocapture transferrin from plasma samples ...
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bioRxiv - Cancer Biology 2023Quote: ... H5B and P62 T cells were cultured in RPMI-1640 (GE Healthcare) supplemented with 10% human AB serum (Institut Jacques Boy ...
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bioRxiv - Neuroscience 2021Quote: ... and transferred onto a methanol-activated PVDF membrane (GE Healthcare) at 60 V for 12 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: Cyanogen bromide (CnBr) activated sepharose 4B lyophilised beads (GE Healthcare) were used according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... was coupled to CNBr-Activated Sepharose 4B beads (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Antigenic proteins were coupled to NHS-activated sepharose (GE Healthcare), whereas antigenic peptides were coupled to SulfoLink coupling gel (ThermoFisher) ...
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bioRxiv - Bioengineering 2020Quote: ... Transmembrane stem cell factor (tmSCF) was then isolated using cobalt chelating column (Chelating column; GE Healthcare), and the buffer was exchanged in 1X PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... before being blotted onto methanol-activated 0.45μm PVDF membranes (GE Healthcare) at 300mA for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare and protein ladder from Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2021Quote: ... and affinity-purified using HiTrap NHS-activated HP columns (GE Healthcare) coupled with His-Dicer-2(1-257)-MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare, SH30027.01) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2019Quote: ... nuclear extracts were first fractionated on High Trap Q (GE Healthcare) prior to Flag-IP ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... unpurified proteins contained in the supernatant of transfected HEK293F cells were immobilized on 2G12 or PGT145 functionalized CNBr-activated Sepharose 4B column (GE Healthcare) by flowing at 1 mL/min or overnight incubation at 4 °C on a roller bank ...
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bioRxiv - Biochemistry 2020Quote: ... and NHS-activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences, IL), was added to the sample ...
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bioRxiv - Microbiology 2020Quote: ... that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare). After extensive washes of the resin with solubilization buffer containing 0.3% (w/v ...
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bioRxiv - Cell Biology 2019Quote: MTED peptide was coupled to cyanogen bromide-activated Sepharose beads (GE Healthcare). 30μM porcine brain tubulin was incubated with either peptide-coated or uncoated Sepharose beads in BRB80 ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biochemistry 2024Quote: ... were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare) and packed into tip columns for the RCC instrument ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescence reaction was activated with ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detection was carried out on a ChemiDoc Imaging System using Image Lab software (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2020Quote: Nb or serum albumin was coupled to CNBr-activated sepharose resin (GE Healthcare). For the in vitro pull-down assay ...
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bioRxiv - Biochemistry 2020Quote: ... GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2021Quote: ... purified superbinder SH2 conjugated to CNBr-activated Sepharose beads (GE Healthcare, Wauwatosa, Wisconsin) was used to capture pTyr containing peptides in our samples as described ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a methanol-activated Amersham Hybond PVDF membrane (GE Healthcare) at 100 V for 1 h at room temperature using Mini Trans-Blot Cell system (Biorad) ...
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bioRxiv - Microbiology 2020Quote: For purification of DUBs cyanogen bromide (CNBr)-activated Sepharose 4B resin (GE Healthcare) was used to couple with Ub-PRG (1.2 pmol ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Neuroscience 2021Quote: ... Discovery MR750 3.0 T (GE Healthcare), MAGNETOM Trio (Siemens Healthcare) ...