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Citations for GE Life Sciences :
1 - 50 of 4830 citations for Rat N Myc Proto Oncogene Protein MYCN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The N protein was then run over a HisTrap FF column (GE Healthcare) in Buffer A (20 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2019Quote: ... N-ChIP chromatin bound to Protein G Sepharose beads (GE Healthcare cat #17-0618-02) were gently washed twice with ice cold 0.5X PBS and spun down for 1 minute at 4°C at 800 rpm ...
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bioRxiv - Microbiology 2021Quote: SEC analysis of purified N protein was performed by Superdex 200 16/600 (GE healthcare) at 4 °C on an AKTA system ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hybond-N+ (GE Healthcare). A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Molecular Biology 2022Quote: Reagents and kits used in this study were: Amersham Hybond N+ nylon membrane (RPN303B, GE healthcare), BCA kit (23225 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was filtered and N-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... α-Myc and α-rabbit horseradish peroxidase conjugate (Amersham, GE Healthcare) were used at dilutions 1:1,000 and 1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Plant Biology 2019Quote: ... Hybond-N+ (GE Healthcare, Chicago, USA), was placed on the MS plate with 1% agar ...
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bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
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bioRxiv - Biochemistry 2021Quote: ... and Amersham Hybond-N+ (GE Healthcare) membranes were equilibrated in 1X membrane wash buffer (20 mM Tris-Cl ...
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bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Proteins were precipitated using the 2D Clean-up Kit (GE Healthcare). The pellet was reconstituted in 50mM ammonium bicarbonate and digested with Trypsin (1/50 w/w ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Immunoreactive proteins were visualized using an ECL prime kit (GE HealthCare).
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of proteins was performed using the ECL kit (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... SEC columns were calibrated with a protein standard kit (GE Healthcare) containing ferritin (MW = 440 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected with ECL Advance kit (GE Healthcare,Poole UK).
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bioRxiv - Immunology 2021Quote: ... blotted onto Hybond-N+ membranes (GE Healthcare) and hybridized to biotin-labeled Sμ and σδ ...
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bioRxiv - Microbiology 2019Quote: ... using Hybond N membranes (Amersham/GE Healthcare) at a hybridization temperature of 45°C.
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bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS)-Sepharose beads (GE Healthcare) were crosslinked with recombinant proteins according to the manufacturer’s instructions and were used for the purification of antibodies against DJ-1 protein ...
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bioRxiv - Developmental Biology 2020Quote: ... transferred onto Hybond N membranes (GE Healthcare), and probed with 32P-random-primed 1kb XmaI-EcoRV cDNA fragment corresponding to the exon 1-4 T-box region.
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bioRxiv - Molecular Biology 2023Quote: ... transferred to Hybond-N+ membrane (GE Healthcare), and hybridized over-night at 50 °C in ULTRA-Hyb Oligo Hybridization buffer (Invitrogen AM8663 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat IgG-HRP (GE Healthcare, UK), anti-rat IgG-HRP (Cell signaling ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...