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Citations for GE Life Sciences :
1 - 50 of 2465 citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: IDH1 R132H: SPR experiments were performed on a Biacore T200 instrument (GE Healthcare) at 25 °C using a running buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... the soluble lysate was loaded onto 1 mL Nickel beads (GE healthcare). The column was washed with lysis buffer containing 15 mM imidazole and his-m6A-Tracer was eluted by 250 mM imidazole in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction was loaded on 1 mL Nickel beads (GE Healthcare). Beads were washed with lysis buffer containing 20 mM imidazole before protein was eluted by 250 mM imidazol in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction was loaded onto a 1-mL anion exchange column (HiTrap 1 mL HP, GE Healthcare) and purified on an Akta purifier system ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded onto a StrepTap HP 1 mL affinity column (GE Healthcare) at 4°C previously equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... 75 μL of soluble thrombin (GE Life Sciences) was added to the dialyzed protein for an overnight incubation at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Soluble extracts were mixed with 1 mL of glutathione agarose beads (catalogue number # 17075601, GE Healthcare) and incubated for 2 h at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Molecular Biology 2021Quote: ... The soluble portion was incubated with glutathione sepharose (GE healthcare) beads for 3-4 hours at 4°C and eluted with glutathione ...
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bioRxiv - Microbiology 2020Quote: ... Soluble proteins were purified using HisTrapTM Excel (GE Healthcare, Poland) column in buffer A with a linear gradient of 0-100% of 1 M imidazole in buffer A in 20 column volumes ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... soluble proteins were applied to a HisTrap-FF column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: Soluble fractions were loaded onto an ÄKTA purification system (GE Healthcare) using an equilibrated HisTrap or GSTrap (both GE Healthcare ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Developmental Biology 2022Quote: ... from which soluble forms were purified on glutathione-Sepharose beads (GE Healthcare). Recombinant C-terminally HA tagged Su(H ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Soluble proteins were purified using GSH-Sepharose 4B (GE Healthcare Life Sciences) and size exclusion chromatography (High Load 16/60 Superdex 200 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was purified using an ÄKTAxpress chromatography system (GE Healthcare). The sample was purified firstly using a 1 mL HisTrap crude column (GE Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction was applied to a HisTrap FF NiNTA column (GE Healthcare) and washed first with high-salt buffer B (2 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble protein was purified by absorption to Ni2+-NTA resin (GE Healthcare), washed with Ni-buffer A (20 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare, USA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was loaded onto a HisTrap HP 5ml column (GE Healthcare), pre-equilibrated with buffer B and then washed with 6 volumes of buffer B supplemented with 30 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: ... The soluble material was purified and loaded on Ni2+-sepharose resin (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... soluble proteins were loaded onto a Ni-column (HisTrap FF Crude, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Biochemistry 2021Quote: ... GST-β2HA was purified from the soluble fraction using glutathione resin (GE Healthcare). The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a 5 ml HisTrap column (GE Healthcare), which was preequilibrated with the Lysis buffer plus 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... the soluble lysate was loaded onto a HiTrap XL SP column (GE Healthcare) for cation exchange chromatography and eluted with a 0-0.5 M NaCl gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with Nickel Sepharose 6 Fast Flow beads (GE Healthcare) for 30 minutes at 4 °C in batch ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was applied to a StrepTrap HP 5 mL column (GE Healthcare) and the bound protein was eluted with Buffer E (Buffer W + 5 mL desthiobiotin) ...
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bioRxiv - Biochemistry 2020Quote: ... CRY2-PHR was isolated from the soluble cell lysate by glutathione sepharose (GE Healthcare) using a buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The soluble fraction was collected and flowed through a HisTrap FF column (GE Healthcare). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... Soluble αReps were purified by affinity chromatography on HisTrap columns (GE Healthcare Life Sciences) and analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction was filtered and loaded 5-ml HisTrap HP column (GE Healthcare) and washed with lysis buffer ...