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Citations for GE Life Sciences :
1 - 50 of 5577 citations for Rat Insulin like growth factor binding protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Free iodine and iodinated growth factor were separated on Sephadex G-25 columns (PD10; GE Healthcare). For column equilibrium and elution ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were loaded in a SuperoseTM 6 Increase (GE Healthcare) column ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Purified protein was then loaded onto a Superose-6 column (GE Healthcare) in 20 mM HEPES pH = 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... Deglycosylated protein was then purified using a Superose 6 column (GE healthcare) equilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by Ni2+-Sepharose 6 Fast Flow (GE healthcare) affinity chromatography with the washing buffer (50mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Biophysics 2020Quote: ... hTRPC5 protein was further purified by size exclusion chromatography (Superose-6, GE Healthcare) in buffer containing TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μL total protein was incubated with 20μL IgG-Sepharose 6 resin (GE healthcare) for 2 hrs at 4°C with rotation ...
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bioRxiv - Biophysics 2022Quote: ... Prior to crystallization the protein was loaded onto a Superose 6 (GE Healthcare) size-exclusion column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... proteins were separated on a Superose 6 Increase 10/300 column (GE Healthcare) in 1x PBS (pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were initially purified using Ni Sepharose 6 Fast Flow resin (GE Healthcare). The protein tags were cleaved with His-tagged TEV-protease overnight at 4 °C while dialyzing against TEV cleavage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated and loaded onto a Superose 6 column (GE Healthcare) pre-equilibrated in purification buffer and peak fractions were pooled and concentrated to 5-6 mg/mL.
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified with Superose 6 Increase 10/300 column (GE Healthcare) (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was passed through a Superose 6 column (GE Healthcare Life Sciences) using gel filtration buffer (20mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was passed through a Superose 6 column (GE Healthcare Life Sciences) using gel filtration buffer (20mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified on a Ni Sepharose 6 Fast Flow column (GE Healthcare) under gravity and eluted with the elution buffer (300 mM Imidazole ...
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... The protein was further purified on a Superose 6 gel filtration column (GE Healthcare) using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently proteins were immobilized on a Ni Sepharose 6 Fast Flow resin (GE Healthcare). The resin was incubated 2-4 h at 4°C with 25 nmol of each fluorophore dissolved in labelling buffer 1 (50 mM Tris-HCl pH 7.4-8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... the L proteins were further purified by size-exclusion chromatography (Superose 6, GE Healthcare) in buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were further purified by SEC (Superose 6 Increase 10/300 GL; GE Healthcare) in a running buffer containing 25 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified using a Superose 6 increase column (GE Healthcare) pre-equilibrated with the binding buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted protein was bound to Ni2+ resin (Ni Sepharose 6 Fast Flow, GE Healthcare) in 6M guanidine HCl ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...