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Citations for GE Life Sciences :
1 - 50 of 78 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: All shRNA’s (GIPZ Lentiviral constructs) were purchased from Dharmacon (GE Healthcare). Pol κ shRNA (RMM4532-EG27015 ...
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bioRxiv - Molecular Biology 2022Quote: ... Inducible Lentiviral shRNA constructs targeting CstF64 were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat IgG-HRP (GE Healthcare, UK), anti-rat IgG-HRP (Cell signaling ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... mouse or rat IgG was purchased from GE Healthcare.
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-linked goat anti-rat IgG pAb (#NA935V, GE Healthcare) and HRP-linked rabbit anti-goat IgG pAb (DAKO #P0449 ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... and anti–α-catenin shRNA (α-cat-KD) (both courtesy of G.D. Longmore, Washington University) were cultured in DMEM/F12 (GE Healthcare Life Sciences), supplemented with 5 % horse serum (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-HRP (GE_NA934V) and anti-rat-HRP (GE_NA935V) (both GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... from the pGEX-6P plasmid (GE Healthcare). Proteins were C-terminally fused to the GST tag and N-terminally to the 3xFLAG tag ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid coding for mouse JNK3 cDNA (GE Healthcare) was used as a PCR template and the resultant product was subcloned into mammalian expression vector pRK5 downstream of a myc epitope tag ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids targeting HNRNPC were purchased from Dharmacon (GE Healthcare). A 2nd generation lentiviral packaging system was used to transfect 293T cells to produce viral particles ...
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bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-coupled secondary antibodies (anti-mouse and anti-rabbit, GE Healthcare; anti-rat, Jackson Immunoresearch) in combination with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Immunology 2022Quote: ... and ECL anti-rat IgG Horseradish Peroxidase linked whole Ab from goat (GE Healthcare UK Limited) were used to detect IgG1 antibodies upon addition of TMB substrate (TMB substrate set ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... the GST sequence from the pGEX-6P plasmid (GE Healthcare) was amplified using primers 5’-GGCAGCAGCCATCATCATCATCATC ACAGCAGCGGCCTGATGTCCCCTATACTAGGTTATTGG-3’ and 5’-TCTTCTTTTGGAACCCA TCATATGGCTGCCGCGCGGCACGGGCCCCTGGAACAGA-3’ ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg Cas9 expression plasmid (GE Healthcare #U-005100-120), and 10 μg repair plasmid (Genscript ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid pGEX-4T1 was purchased from GE Healthcare (Cat #: 28-9545-49). Plasmid pET His6 Sumo TEV LIC (Addgene # 29659 ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAi efficiency testing for ELAV was done from dissected central brains by Western blotting according to standard protocols as described 73 using a polyclonal rat anti-ELAV antibody generated against Drosophila ELAV (1:800) 45 and secondary HRP-coupled goat anti-rat antibody (1:5000, GE Healthcare) by chemoluminescence detection (Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... a PCR fragment containing ubiquitin gene and a PCR fragment containing rat parkin residues 77-465 were introduced into the BamHI/XhoI sites of pGEX-6P1 (GE Healthcare). The serine (S77 ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...
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A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activitybioRxiv - Biochemistry 2019Quote: DNA substrates were prepared by diluting plasmid DNA in HyClone water™ (GE healthcare), and unmodified HPLC-purified oligonucleotide substrates (Eurofins ...
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bioRxiv - Genetics 2022Quote: ... All purified plasmids were quantified in NanoVue PlusTM (GE Healthcare, Princeton, NJ, United States) and a final concentration of 50 ng/μL plasmids was used for hybridization ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells from the pDYH24 plasmid and purified with glutathione sepharose (GE Healthcare). PUD-1/PUD-2 heterodimers were purified on a HisTrap column followed by gel filtration ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into the BamHI and EcoRI sites of the pGEX-2T plasmid (GE Life Sciences) for in frame translation with glutathione S-transferase (GST) ...
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bioRxiv - Molecular Biology 2021Quote: ... Paris) by first amplifying plasmid mini preps with the Illustra TempliPhi Large Construct Kit (GE Healthcare) and subsequently labelling 2 μg of the amplification product by nick translation using Green UTP or Red UTP (Abbott Molecular ...