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Citations for GE Life Sciences :
1 - 50 of 669 citations for QuantiChrom β Glucosidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM β-mercaptoethanol) on an ÅKTA system (GE Healthcare). PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... mouse anti-β-tubulin 1:8000 (13-8000, GE Healthcare), mouse anti-Repo 1:20 (8D12 ...
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bioRxiv - Biophysics 2019Quote: A pET-based plasmid overexpressing each subunit of RNAP (full-length α, β, ω) as well as β’-PPX-His10 (PPX; PreScission protease site, LEVLFQGP, GE Healthcare) was co-transformed with a pACYCDuet-1 plasmid containing Eco rpoZ into Eco BL21(DE3) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM β-mercaptoethanol) using gel-filtration NAP column (GE Healthcare) and concentrated by ultrafiltration to 1.2 ...
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bioRxiv - Neuroscience 2021Quote: ... air dried and exposed to Hyper film β-max (GE Healthcare, USA) for seven days ...
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bioRxiv - Synthetic Biology 2022Quote: ... and PAA-β-galactose (Lectinity) were immobilized on CM5 chips (GE Healthcare) pre-coated with streptavidin ...
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bioRxiv - Biophysics 2021Quote: ... β-catenin was further purified using a Mono-Q column (GE Life Sciences) equilibrated in 50 mM Tris-HCl buffer pH 8.9 ...
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bioRxiv - Biophysics 2023Quote: ... and 0.5% β-ME) and exposed for radiography (Typhoon Biomolecular Imager, GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Cancer Biology 2021Quote: ... An electrochemiluminescence assay (GE Healthcare) was then performed and signals were acquired using a ChemoCam Imager (Intas ...
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bioRxiv - Molecular Biology 2021Quote: ... β-gal was purified using Superdex 200 Increase 10/300 GL column (GE Healthcare) with the same buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Physiology 2021Quote: ... β-aminopropionitrile (400 μM) and 2.5 μCi/ml [14C]proline (GE Healthcare, Illinois, USA) and incubated at 37°C for 18 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction was quenched with β-mercaptoethanol and desalted using PD-10 columns (GE healthcare). Extent of reaction was checked using 1D intact weight ESI mass spectrometry (SIRCAMs ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl and 4 mM β-mercaptoethanol using an Akta FPLC system (GE Healthcare). Pure His-tev-ecMSG or tag-cleaved ecMSG was concentrated to 40-80 mg/ml ...
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bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Biochemistry 2019Quote: ... 5% (vol/vol) glycerol and 5 mM β-mercaptoethanol using a HiTrap Desalting column (GE Healthcare) and the GST tag was cleaved with GST-TEV protease overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol) and further purified by gel filtration over a Superdex-200 column (GE Healthcare). The protein product was concentrated with a centrifugal filter unit (MD Millipore ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM β-mercaptoethanol and loaded onto a 1 ml MonoQ anion exchange chromatography column (GE Healthcare). After washing with 10 column volumes of buffer ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.0 and 5 mM β-mercaptoethanol) containing 100 mM NaCl by using desalting column (GE Healthcare) and further purified by affinity chromatography with HiTrap heparin column (GE Healthcare) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with ∼650 μM β-mercaptoethanol and desalted using PD-10 columns (GE healthcare). The extent of reaction was checked using 1D intact weight ESI mass spectrometry (SIRCAMs ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μM ZnCl2 and 5 mM β-mercaptoethanol) for XPA and Superdex 200 10/300 increase (GE Healthcare) equilibrated in a different storage buffer (400 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were measured using BCA assay (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Immunology 2020Quote: ... the presence of β-actin was revealed (clone 8H10D10) Development was performed with ECL Western blotting reagents (GE Healthcare, UK).
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bioRxiv - Molecular Biology 2021Quote: ... and the assay buffer was HBS-EP+ buffer (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... ITC binding assay was performed via an ITC200 (GE Healthcare) equipment at 25 °C ...
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bioRxiv - Microbiology 2023Quote: Column-based binding assay used heparin-agarose beads (GE Healthcare) as described (48 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 5-fold with buffer A (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A supplemented with 100 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 3-fold with dilution buffer (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Pol II buffer was exchanged to CB buffer (25mM HEPES pH 7.9, 100mM NaCl, 1mM MgCl2 and 1mM β-mercaptoethanol) and concentrated with Vivaspin 50kDa MWCO (GE Healthcare). Pol II was aliquoted and snap-frozen in liquid nitrogen until the day of the experiment.
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Assays were performed in 50% HBS-EP (GE Healthcare Life Sciences) plus 50% Nano-Glo assay buffer ...
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bioRxiv - Microbiology 2020Quote: Surface plasmon resonance assay using a Biacore S200 system (GE Healthcare) was carried out as previously described (20) ...
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bioRxiv - Biochemistry 2023Quote: ... All gel-based assays were analyzed using ImageQuant 5.2 (GE Healthcare) and all data fitting was performed using Kaleidagraph.
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bioRxiv - Immunology 2023Quote: SPR competition assays were performed on a Biacore 4000 (GE Healthcare) in 10 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mg of β-galactosidase G5635-5KU were independently applied to a Superdex 200 10/300 GL analytical gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES KOH ...
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bioRxiv - Cell Biology 2022Quote: ... The Glo3 BoCCS and GRM regions and β’-COP (residues 300-604) were ligated into BamHI/ NotI sites of pGEX-6P-1 (GE Healthcare), resulting in an N-terminal GST-tagged proteins with a 3C-protease cleavage site ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.