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Citations for GE Life Sciences :
1 - 50 of 656 citations for Pyruvate Kinase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Immunology 2019Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Physiology 2019Quote: ... supplemented with 1 mM sodium pyruvate (GE Healthcare, Thermo-Fisher), 1% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Neuroscience 2019Quote: ... were measured on a 96-well microplate (Biacore, GE Healthcare) at 25 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... using 96 well filter plates (Whatman Polystyrene Unifilter Microplates, GE Healthcare, 11313535). After purification ...
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bioRxiv - Biochemistry 2021Quote: ... [γ-32P] (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Fischer) and subsequently purified using Illustra Microspin G25 columns (GE healthcare). Recombinant EB1 (0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Molecular Biology 2023Quote: ... The amount of [33Pγ]-tracer on the paper (signifying the kinase activity) was analyzed using a Typhoon FLA 700 IP PhosphorImager (GE Healthcare, Denmark), and related to the specific activity of the kinase reaction buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Cancer Biology 2021Quote: ... An electrochemiluminescence assay (GE Healthcare) was then performed and signals were acquired using a ChemoCam Imager (Intas ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA yield was quantified using a NanoVue microplate reader (GE Healthcare, Chicago, IL, USA). The sample volume was adjusted to 2 µg/100 µL for subsequent RNA-seq analysis ...
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bioRxiv - Bioengineering 2023Quote: ... Further purification was done in 96 well microplates using Capto Q AEX-resin (GE Healthcare).
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2021Quote: ... They were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – #SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and were cultured in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were measured using BCA assay (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... and the assay buffer was HBS-EP+ buffer (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... ITC binding assay was performed via an ITC200 (GE Healthcare) equipment at 25 °C ...
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bioRxiv - Microbiology 2023Quote: Column-based binding assay used heparin-agarose beads (GE Healthcare) as described (48 ...
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bioRxiv - Molecular Biology 2021Quote: ... Assays were performed in 50% HBS-EP (GE Healthcare Life Sciences) plus 50% Nano-Glo assay buffer ...
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bioRxiv - Microbiology 2020Quote: Surface plasmon resonance assay using a Biacore S200 system (GE Healthcare) was carried out as previously described (20) ...
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bioRxiv - Biochemistry 2023Quote: ... All gel-based assays were analyzed using ImageQuant 5.2 (GE Healthcare) and all data fitting was performed using Kaleidagraph.
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bioRxiv - Immunology 2023Quote: SPR competition assays were performed on a Biacore 4000 (GE Healthcare) in 10 mM HEPES (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... SPR based assays were performed using the Biacore™ T200 (GE Healthcare) system and the kinetic rate constants (ka and kd ...
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bioRxiv - Microbiology 2021Quote: ... All assays were performed with a Sensor Chip Protein A (GE healthcare),with a HBS EP+ running buffer (0.1M HEPES ...
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bioRxiv - Microbiology 2022Quote: ... Plaque assay analysis was performed using ImageQuant TL 8.2 software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Plaque assay analysis was performed using ImageQuant TL 8.2 software (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: SPR assays were carried out using a BIAcore T200 instrument (GE Healthcare). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... All assays were performed using a Sensor Chip Protein A (GE Healthcare), with a running buffer of PBS pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Plaque assay analysis was performed using ImageQuant TL 8.2 software (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: Surface plasmon resonance assay was performed using Biacore 8k (GE Healthcare, USA). The purified proteins or domains were immobilized on CM5 chip (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: All surface plasma resonance assays were performed on a Biacore 3000 (GE Healthcare) with a running buffer of 10 mM HEPES pH 7.5 and 150 mM NaCl supplemented with 0.05% Tween 20 at 25°C ...