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Citations for GE Life Sciences :
1 - 50 of 863 citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Plant Biology 2022Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 µl 0.5 mM NiCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2020Quote: ... or 10% FCS fetal clonal serum (FCS)(HyClone, GE Life Sciences), 100 U/ml penicillin and streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... FCS (SH30071.03) from GE Healthcare Life Sciences and Puromycin from SIGMA (P9620) ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS (GE Healthcare) and antibiotics (100 U ml−1 penicillin and 100 µg ml−1 streptomycin) ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Immunology 2021Quote: ... 10% (v/v) FCS (Hyclone, GE Healthcare) and 0.1 mg/ml penicillin/streptomycin (15140122 ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... Fc was removed via ProteinA Sepharose incubation (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% fetal calf serum (FCS, GE Healthcare Life Sciences) and 500 U/mL penicillin-streptomycin (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Fc-fusion proteins in HBS-EP+ buffer (GE Healthcare) were immobilized at a capture level of 600-800 response units (RU ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare), 100 µg/mL of streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% ultra-low IgG FCS (GE Healthcare), 100 μg/mL of Gentamycin ...
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bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The 6xHis-Lipo tag was cleaved off using Thrombin (GE Healthcare) and the protein was separated from the cleaved tag using the same column and buffers as described above ...
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bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Cell Biology 2019Quote: ... followed by Step-tag affinity chromatography using StepTactin resin (GE Healthcare) in BRB20 ...
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bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS; Hyclone, GE Healthcare), 50 μM 2-mercaptoethanol ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200-1500 response units (RUs) ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200 -1500 response units (RUs) ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... the cleaved MBP tag was removed with an MBPTrap column (GE Healthcare), and the protein was bound to a HiTrap heparin HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... Fc conjugated protein concentration was quantified using NanoVue Spectrophotometer (GE Healthcare).