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Citations for GE Life Sciences :
1 - 50 of 6288 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The amount of [33Pγ]-tracer on the paper (signifying the kinase activity) was analyzed using a Typhoon FLA 700 IP PhosphorImager (GE Healthcare, Denmark), and related to the specific activity of the kinase reaction buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... HRP activity was detected using the ECL detection system kit (GE Healthcare) and the Amersham Imager 680 (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were measured using BCA assay (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Biochemistry 2019Quote: ... The peroxidase activity was revealed using an enhanced chemiluminescence detection kit (GE Healthcare, # 16915806). GAPDH antibody (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... The outcome of the activity assay was visualized with a fluorescent scan of an SDS-PAGE gel with Typhoon imager (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... All assays were performed with a Sensor Chip Protein A (GE healthcare),with a HBS EP+ running buffer (0.1M HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... All assays were performed using a Sensor Chip Protein A (GE Healthcare), with a running buffer of PBS pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The different phosphorylation states of PKA-CF100A were separated using a cation exchange column (HiTrap Q-SP, GE Healthcare Life Sciences) using a linear gradient of KCl in 20 mM KH2PO4 at pH6.5.83 The purified protein isoforms were then stored in phosphate buffer containing 10 mM dithiothreitol (DTT) ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of peroxidase activity was performed by using the Amersham ECL western blot detection kit (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The gels were exposed to imaging plates and the images of separated tracer-labeled proteins were obtained by reading the plate using a Typhoon 9410 (GE Healthcare) image analyzer ...
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... 10(n)-3H-oleic acid (specific activity, 45.5 Ci/mmol) and [4-14C]-cholesterol (specific activity, 49.8 mCi/mmol) were purchased from GE Healthcare (Piscataway, NJ, USA). 1-14C-oleic acid was obtained from Moravek Biochemicals Inc ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... Peroxidase activity was developed in ECL Plus reagent (GE Healthcare) and chemiluminescence signal captured with the LAS-4000 imaging system (Fujifilm).
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bioRxiv - Plant Biology 2022Quote: ... HRP activity was detected by enhanced chemiluminescence (ECL, GE Healthcare) according to the manufacturer’s instructions using either a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Enzyme activity was developed using ECL prime reagent (GE Healthcare) on ChemiDoc XRS+ imaging system.
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bioRxiv - Plant Biology 2024Quote: ... HRP activity was detected using enhanced chemiluminescence (ECL; GE Healthcare) according to the manufacturer’s instructions with either ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... HRP activity was detected by enhanced chemiluminescence (ECL, GE Healthcare) according to the manufacturer’s instructions using either a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Proteins were precipitated using the 2D Clean-up Kit (GE Healthcare). The pellet was reconstituted in 50mM ammonium bicarbonate and digested with Trypsin (1/50 w/w ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Immunoreactive proteins were visualized using an ECL prime kit (GE HealthCare).
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of proteins was performed using the ECL kit (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... SEC columns were calibrated with a protein standard kit (GE Healthcare) containing ferritin (MW = 440 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected with ECL Advance kit (GE Healthcare,Poole UK).
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Peroxidase activity was detected using Western Lightning Chemiluminescence Reagent (GE Healthcare). The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... Peroxidase activity was detected using Western Lightning Chemiluminescence Reagent (GE Healthcare). The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... HRP activity was detected using Western Lightning Chemiluminescence Reagent (GE Healthcare). The molecular migration position of transferred proteins was compared with the PageRuler Prestain Protein Ladder 10– 180 kDa (Themo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Molecular Biology 2024Quote: ... fritted deep-well plates were used containing 25 μl of settled Protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Bioengineering 2022Quote: ... gels were either silver stained (PlusOne Silver Staining Kit, Protein; GE Healthcare) or transferred onto nitrocellulose membranes for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated using the 2D Clean Up Kit (GE Healthcare) according to the manufacturer’s instructions ...