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Citations for GE Life Sciences :
1 - 50 of 1402 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cardiac function was assessed using cardiac echography under general anesthesia (ViVid i; GE Healthcare, WI, USA) with an 11.5-MHz transducer every week until 4 weeks after implantation ...
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bioRxiv - Physiology 2024Quote: ... a Vivid E90 cardiac ultrasound system (GE Healthcare) using an L8-l8i-D transducer ...
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bioRxiv - Bioengineering 2023Quote: ... with an 8-channel cardiac phased-array coil (GE Healthcare, Waukesha, WI). Volunteers performed tidal breathing in a supine position ...
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bioRxiv - Immunology 2021Quote: ... Cardiac function was measured by Vivid 7 ultrasound machine (GE Healthcare, Pittsburgh, PA) and 13-MHz linear array transducer ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Physiology 2019Quote: Cardiac dimensions and contractility were assessed by right para-sternal transthoracic echocardiography (Vivid 7; GE Healthcare, UK) in non-sedated conscious sheep gently restrained in a supine position ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary CLL cells were grown in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin at 37°C and 5% CO2.
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bioRxiv - Immunology 2023Quote: ... 6 and 14 we euthanized 5 individuals per group and performed an intra-cardiac terminal blood collection for PBMCs isolation with Ficoll-Paque Plus (GE healthcare). We performed a complete necropsy of all animals and collected samples of the lungs ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed asexual blood stage parasites collected from an infected mouse by cardiac puncture were filtered through CF11 cellulose columns (Whatman-GE Healthcare Life Sciences) to exclude mice white blood cells and immediately lysed with ice-cold erythrocyte lysis buffer (1.5M NH4Cl ...
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bioRxiv - Physiology 2020Quote: ... transthoracic echocardiography was performed for cardiac examination using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France) as described in the supplemental methods herein ...
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bioRxiv - Cell Biology 2019Quote: ... Cardiac sections processed for immunofluorescence were imaged on a Leica SP5 confocal microscope or a widefield deconvolution microscope (DeltaVision; Applied Precision/GE Healthcare) with a charge-coupled device (CCD ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from primary donors using Ficoll-Paque Plus (GE Life Sciences) and frozen until use ...
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bioRxiv - Molecular Biology 2019Quote: Primary CD4+ T cells were isolated from buffy coats from healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCells Technologies) ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Cancer Biology 2021Quote: Primary BCP-ALL and normal bone marrow mononuclear cells were purified using Ficoll (#17144002, GE Healthcare, Pittsburgh, PA). Human specimen collection protocols were reviewed and approved by Children’s Hospital Los Angeles Institution Review Board (IRB ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats using Ficoll-Paque PLUS (GE Healthcare Bioscience). The PBMCs were cryopreserved in 40% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cancer Biology 2023Quote: ... Primary CAFs were cultured in RPMI-1640 (GE Healthcare) supplemented with 10% human AB serum (Institut Jacques Boy ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary CAFs were cultured in RPMI-1640 (GE Healthcare) supplemented with 10% human AB serum (Institut Jacques Boy ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).