Labshake search
Citations for GE Life Sciences :
1 - 50 of 1410 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Brain cells isolated were washed and subjected to 30% Percoll (GE Healthcare) density gradient centrifugation at 500g for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: ... were added at 25ul/ml to exclude immune and endothelial cells using MagRack 6 (GE Healthcare 89129-096). Cells were either frozen for future use or stained for flow cytometry ...
-
bioRxiv - Neuroscience 2019Quote: ... with 2% FBS and 0.5 mg/mL DNase I) and endothelial cells were obtained using density gradient centrifugation with 22% Percoll (GE Healthcare) in PBS (1X) ...
-
bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
-
bioRxiv - Immunology 2021Quote: ... Immune cells were isolated from brains and spinal cords using a 30% Percoll gradient (GE Healthcare) and were resuspended in Flow Cytometry Staining Buffer (FACS buffer ...
-
bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
-
bioRxiv - Immunology 2020Quote: Brain tissue from the animals was collected and NK cells were isolated using a percoll (GE Healthcare) gradient fallowing protocol described by Pino et al 57 ...
-
bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary CLL cells were grown in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin at 37°C and 5% CO2.
-
bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
-
bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
-
bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
-
bioRxiv - Neuroscience 2021Quote: Total RNA from brain tissues or culture cells were extracted using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare Life Sciences) for reverse transcription with the High-Capacity cDNA Reverse Transcription Kits (ABI Applied Biosystems ...
-
Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
-
bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
-
bioRxiv - Bioengineering 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from primary donors using Ficoll-Paque Plus (GE Life Sciences) and frozen until use ...
-
bioRxiv - Molecular Biology 2019Quote: Primary CD4+ T cells were isolated from buffy coats from healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCells Technologies) ...
-
bioRxiv - Neuroscience 2022Quote: 3D T1-weighted brain volumes were acquired at 1.5 Tesla (Signa, GE Healthcare) using a 3D Magnetization-Prepared Gradient-Echo BRAVO sequence (TR/TE/TI 8.2/3.1/450 ms ...
-
bioRxiv - Biophysics 2022Quote: Tubulin from bovine brain (PurSolutions) was labeled with Cy5 NHS ester (GE Healthcare) as previously described51.
-
bioRxiv - Neuroscience 2021Quote: 3D T1-weighted brain volumes were acquired at 1.5 Tesla (Signa, GE Healthcare) using a 3D Magnetization-Prepared Gradient-Echo BRAVO sequence (TR/TE/TI 8.2/3.1/450 ms ...
-
bioRxiv - Neuroscience 2022Quote: ... 3D T1-weighted brain volumes were acquired at 1.5 Tesla (Signa, GE Healthcare) after the MEG recording (14) ...
-
bioRxiv - Neuroscience 2023Quote: ... The brain slices were imaged with DeltaVision Ultra automated widefield microscope (GE Healthcare).
-
bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
-
bioRxiv - Cancer Biology 2021Quote: Primary BCP-ALL and normal bone marrow mononuclear cells were purified using Ficoll (#17144002, GE Healthcare, Pittsburgh, PA). Human specimen collection protocols were reviewed and approved by Children’s Hospital Los Angeles Institution Review Board (IRB ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
-
bioRxiv - Immunology 2023Quote: ... Brain homogenates were resuspended in 70% percoll (in PBS; GE Healthcare, CA, Illinois, USA) layered under 37% percoll solution topped by a 30% percoll solution in a 15 mL Falcon tube and immediately centrifuged 30 min (acceleration of 2 and deceleration of 1) ...
-
bioRxiv - Neuroscience 2024Quote: Whole-brain imaging was performed using a 3T GE scanner (GE HealthCare, United States) with a 16-channel head coil ...
-
bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
-
bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
-
bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats using Ficoll-Paque PLUS (GE Healthcare Bioscience). The PBMCs were cryopreserved in 40% heat-inactivated fetal bovine serum (FCS ...
-
bioRxiv - Neuroscience 2019Quote: Whole brain images were acquired with the 3 T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin-Madison ...
-
Cognitive and neural bases of decision-making causing civilian casualties during intergroup conflictbioRxiv - Neuroscience 2020Quote: Brain images were acquired using a 3.0T GE Signa MR750 scanner (GE Healthcare; Waukesha, WI) with a standard 8 channel head coil ...
-
bioRxiv - Neuroscience 2023Quote: Whole brain images were acquired with a 3-T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison ...
-
bioRxiv - Bioengineering 2023Quote: Tubulin from bovine brain (PurSolutions) was also labeled with Alexa 568 NHS ester (GE Healthcare) as previously described(58).
-
bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
-
bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
-
bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
-
bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
-
bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
-
bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
-
bioRxiv - Biophysics 2019Quote: Porcine and bovine brain tubulin was labelled with Cy5-or Alexa-488 NHS ester (GE Healthcare) to 54% labelling using previously described methods (Gell et al. ...
-
bioRxiv - Immunology 2021Quote: The procedure for the isolation of brain-infiltrating leukocytes (BILs) using Percoll (GE Healthcare Life Sciences) was previously described.17 To isolate tumor-infiltrating leukocytes (TILs) ...
-
bioRxiv - Neuroscience 2022Quote: Whole-brain imaging data were acquired on a 3T GE Discovery MR750 MRI scanner (GE Healthcare) using a 32-channel radiofrequency receive-only head coil (Nova Medical) ...