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Citations for GE Life Sciences :
1 - 50 of 5522 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... 4°C (SORVALL RC 6+) and supernatant was applied to a HisTrap affinity column (GE Healthcare) that was pre-equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on film (Kodak ...
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bioRxiv - Genetics 2023Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on G:BOX gel imaging system (Syngene ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Neuroscience 2022Quote: ... for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for rabbit antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody binding was detected by enhanced chemiluminescence (GE Healthcare; Buckinghamshire, United Kingdom). Protein quantification was performed using a GS-900 Calibrated Densitometer (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2019Quote: ... All poly-histidine tagged proteins were purified using the Ni-NTA agarose (GE Healthcare), and eluted with imidazole ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected by binding goat anti-rabbit IgG-HRP (NA934, GE Healthcare), goat anti-mouse IgG-HRP (NA931 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was followed by anti-mouse (GE Healthcare, catalog # NXA931V, lot # 17041890) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Primary antibody binding was followed by anti-mouse-IgG (GE Healthcare, catalog #NXA931V, lot #17193521) or anti-rabbit-IgG (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Immunology 2020Quote: ... The binding of primary antibody was detected by secondary antibodies and observed by GE Amersham Imager 600 Imaging System (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Antibody binding was visualized with Amersham ECL Western blotting detection reagent (GE Healthcare, Little Chalfont, UK).
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bioRxiv - Cell Biology 2023Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Genetics 2023Quote: ... Protein G Sepharose 4 Fast Flow (GE Healthcare, 17061801) was equilibrated with PBS containing 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...