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Citations for GE Life Sciences :
1 - 50 of 2595 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: Radioactive nucleotides were from Perkin Elmer and unlabeled nucleotides were from GE Healthcare. DNA modification enzymes were from New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... Free-unlabeled dye was removed by PD-10 columns (GE healthcare) and concentrated using Amicon® Ultra Centrifugal Filters (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... The unlabeled fluorophores were separated using PD-10 columns (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT). MCF7 cells used were authenticated to be 100% match to MCF7 cells (ATCC ...
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bioRxiv - Cancer Biology 2021Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT) at 37 °C in 5% CO2 ...
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bioRxiv - Pathology 2019Quote: ... 5ug/mL Polyadenylic acid (GE Healthcare 27-4110-01), 2.5ug/mL pd (t)12-18 (Oligo dT ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 U/ml penicillin and 100 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences). All cells were routinely cultured in a humidity incubator at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... designs (labeled and unlabeled) were further purified by size-exclusion chromatography on ÄKTAxpress (GE Healthcare) using a Superdex 75 10/300 GL column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 U/mL penicillin/0.1 mg/mL streptomycin (GE Healthcare, USA), 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Immunology 2019Quote: ... 100 U/mL of Penicillin (GE Healthcare, catalog SV30010), and 100 µg/mL of Streptomycin (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... and 100 U/mL HyClone penicillin-streptomycin (GE Healthcare) at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Biochemistry 2021Quote: ... All labeled and unlabeled G-actins were further purified by size-exclusion chromatography on Sephacryl S200-HR (GE Healthcare, Chicago, IL), stored on ice in G-buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was stopped on ice and labeled polyP300-AF647 was separated from free dye and unlabeled polyP via a NAP-5 column (GE Healthcare) that was equilibrated with 40 mM KPi ...
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bioRxiv - Immunology 2019Quote: ... and 100 µg/mL of Streptomycin (GE Healthcare, catalog SV30010)) at 37 °C/5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... and 100 mg/mL streptomycin (HyClone, GE Healthcare Life Sciences), at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 ml of 100 kU DNase I (GE healthcare) for 10 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension volume was adjusted to 33.4 mL with PBS and 10 mL of 100% isotonic Percoll (9 mL Percoll PLUS (GE Healthcare), 1 mL 10x PBS without Ca and Mg ...
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bioRxiv - Cancer Biology 2023Quote: ... Patients were injected with 100–120 mL iohexol (Omnipaque, GE Healthcare) at an injection rate of 4–5 mL/s ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with 1.2 ml of fetal bovine serum (FBS) and 3.6 ml of 100% Percoll (GE Healthcare). The Percoll cell suspension was overlaid with 1 ml of 10% FBS in DMEM and spun at 800g for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Immunology 2020Quote: ... and penicillin (100 U/mL) (RPMI++) were transduced with filtered (0.45 μm) and concentrated (100 kDa molecular weight cutoff, GE Healthcare) HEK 293T supernatant ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was cleaved off by incubating the washed beads in wash buffer supplemented with PreScission Protease (∼100 mL at 76 mg/mL) (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates 30-50 ug were run on SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). Western blots were conducted using anti-CTLA-4/CD152 (LS C193047 ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was neutralized with 2 M Tris pH7.0 and either diluted first 1:1 with 20 mM phosphate pH7.0 buffer (25 mL milk sample) or loaded directly (100 mL milk sample) onto a Q Sepharose FF (GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM phosphate pH7.2 buffer column at 150 cm/h and 90 cm/h ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2019Quote: ... 500 mM Imidazole) (0-100% over 50 ml) on an ÄKTA Pure (GE healthcare, USA) at 2 ml/min ...
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bioRxiv - Immunology 2021Quote: ... the transfected cells were supplemented with 100 ml of fresh HyClone SFM4HEK293 medium (GE Healthcare) and sodium butyrate (Reeves et al. ...
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bioRxiv - Plant Biology 2022Quote: ... 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads, GE Life Sciences) were added to each sample in 50% ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Microbiology 2019Quote: Purified capsids (100 µg) were loaded onto a 5-ml HiTrap™ Heparin-Sepharose HP Column (GE Healthcare), which then washed with Ca2+ and Mg2+-free PBS to remove unbound molecules and eluted with gradually linear increasing concentrations of NaCl ...