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Citations for GE Life Sciences :
1 - 50 of 628 citations for PD L1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... anti-PD-L1 and LH02 were all purified by affinity chromatography using a protein A affinity column (GE Healthcare, Piscataway, NJ, USA) and analyzed in reducing condition on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Cell Biology 2020Quote: ... The L1 chip (GE Healthcare) was coated with composite liposomes (43% cholesterol / 16% sphingomyelin / 17% POPC / 12% POPS / 9% POPE / 1% ceramide and 2% POPA or 14% POPS / 0% POPA for background reference and equally charged liposomes) ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Biophysics 2023Quote: ... PD-10 columns (GE Healthcare) were used for buffer exchange to 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... 0.150 M NaCl) using PD-10 or PD-MidiTrap G-25 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Biochemistry 2021Quote: PD-10 Desalting Columns (GE Healthcare)
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bioRxiv - Biochemistry 2021Quote: ... PD- Minitrap G-25 (GE Healthcare) columns were used to desalt and exchange the buffer to 20 mM Tris (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 column (GE Healthcare) was used to remove the βME ...
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bioRxiv - Microbiology 2023Quote: ... using PD-10 columns (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Microbiology 2022Quote: ... L1 chips for SPR experiments were purchased from GE Healthcare. For cell viability assays ...
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bioRxiv - Cell Biology 2019Quote: ... a PD-10 desalting column (GE Healthcare) and a concentrator (Vivaspin MWCO 10K ...
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bioRxiv - Biochemistry 2022Quote: ... using a PD-10 column (GE Healthcare). Mad1 and GST-Mps1 were mixed at a 5:1 ratio ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Plant Biology 2020Quote: ... desalted on a PD-10 column (GE Healthcare) equilibrated with 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2022Quote: ... After column mediated desalting (PD-10, GE Healthcare), fractions were exchanged to a buffer containing 10 mM Tricine (pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... A PD MiniTrap G-25 column (GE Healthcare) was used to remove imidazole ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Biophysics 2021Quote: ... Using a PD-10 desalting column (GE healthcare) buffer was exchanged to storage buffer (50 mM Tris pH 8.3 ...
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bioRxiv - Biophysics 2020Quote: ... using a PD-10 desalting column (GE Healthcare). Protein after PD-10 was labeled overnight at 4°C by immediately mixing with a 10-fold molar excess of the spin label (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol using PD-10 columns (GE healthcare). If required YpfP was concentrated using a Vivaspin 20 30MWCO until 2.4 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... using a PD-10 desalting column (GE Healthcare). Purified Ndc80C was stored at −80°C.
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bioRxiv - Cell Biology 2021Quote: ... and PD-10 (GE Healthcare, 17-0851-01) desalting columns ...
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bioRxiv - Biochemistry 2023Quote: ... and PD-10 columns (GE Healthcare, Uppsala, Sweden) were used for buffer exchange with storage buffer 20 mM HEPES pH 7.5 and 50 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and desalting (PD 10 Desalting Columns, GE Healthcare), the purified VitR protein was resolved by 15% SDS-PAGE.
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bioRxiv - Biochemistry 2020Quote: ... and Sharpin were eluted and further purified over a Superdex 75 16/600 pd or a Superdex 200 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... or 10% FCS fetal clonal serum (FCS)(HyClone, GE Life Sciences), 100 U/ml penicillin and streptomycin (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Desalting was conducted using PD-10 column (GE healthcare) following manufacture’s protocol.
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bioRxiv - Plant Biology 2019Quote: ... pH 7.9 by PD-10 Desalting Columns (GE Healthcare). The 6xHis-Tag was removed by overnight digestion with thrombin protease ...
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bioRxiv - Biochemistry 2019Quote: ... using a PD-10 desalting column (GE Life Sciences). Protein was loaded onto a MonoQ anion exchange column (GE Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) using PD-10 desalting columns (GE Healthcare). Buffer exchanged protein was stored in aliquots at -80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were applied to PD-10 columns (GE Healthcare) to remove residual Zn2+ ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4) using PD-10 desalting columns (GE Healthcare), and stored at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... purified using a PD 10 desalting column (GE Healthcare) to remove excess PSMA and stored in pH 9 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 desalting column (GE Healthcare, Chicago, IL) was used to remove excess imidazole and buffer exchange in between the Ni affinity purification and thrombin cleavage ...
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bioRxiv - Biophysics 2023Quote: ... 0.1% DDM using PD-10 Desalting column (GE Healthcare). The desalted protein was immediately mixed with 40-fold excess of the spin label ...
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bioRxiv - Neuroscience 2023Quote: ... then loaded on a PD-10 column (GE healthcare). The columns were washed with buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The 2.5 ml of R-β-actin sample was desalted to remove DTT leftovers by using PD-10 (Prepacked Disposable PD-10 Columns, GE Healthcare #17085101) column pre-equilibrated with G-buffer without DTT ...
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bioRxiv - Evolutionary Biology 2019Quote: ... pH 7.4) using disposable PD-10 desalting columns (GE Healthcare), sterilized through a 0.22 µm syringe filter ...
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bioRxiv - Biochemistry 2019Quote: ... desalted using a PD SpinTrap G-25 column (GE Healthcare) and concentrated using an Amicon Ultra-0.5 device (MW cut-off 10 kDa) ...
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bioRxiv - Physiology 2019Quote: ... Adenoviruses were purified by PD-10 column (17085101, GE Healthcare). Titers were determined by plaque assay (PFU) ...
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bioRxiv - Bioengineering 2019Quote: ... PD MidiTrap G-25 column were obtained from GE Healthcare Life Sciences (Basel ...
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bioRxiv - Cell Biology 2019Quote: ... 50μM ATP) using PD-10 desalting columns (GE Healthcare # 17085101). Protein was then digested with TEV protease and run through a subtractive Ni-NTA column to remove TEV protease and uncut protein ...
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bioRxiv - Immunology 2021Quote: ... Desalting column (PD-10) was purchased from GE life sciences, India ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated proteins were desalted using PD-10 columns (GE Healthcare) and exchanged into BRB80 buffer (80 mM piperazine-1,4-bis(2-ethanesulfonic acid) ...