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Citations for GE Life Sciences :
1 - 50 of 2685 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM TCEP) using PD-10 Sephadex desalting column (GE Healthcare). The following samples (50 μL final reaction volume ...
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bioRxiv - Biophysics 2023Quote: ... PD-10 columns (GE Healthcare) were used for buffer exchange to 50 mM Tris-HCl pH 7.5 ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... 0.150 M NaCl) using PD-10 or PD-MidiTrap G-25 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Biochemistry 2021Quote: PD-10 Desalting Columns (GE Healthcare)
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bioRxiv - Biochemistry 2021Quote: ... PD- Minitrap G-25 (GE Healthcare) columns were used to desalt and exchange the buffer to 20 mM Tris (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 column (GE Healthcare) was used to remove the βME ...
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bioRxiv - Microbiology 2023Quote: ... using PD-10 columns (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cell Biology 2019Quote: ... 1 mM EGTA) supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare). Drosophila KLP10A (plasmid pGG885 ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Cell Biology 2019Quote: ... a PD-10 desalting column (GE Healthcare) and a concentrator (Vivaspin MWCO 10K ...
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bioRxiv - Biochemistry 2022Quote: ... using a PD-10 column (GE Healthcare). Mad1 and GST-Mps1 were mixed at a 5:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... After 1 hour the excess dye was removed via PD-10 desalting column (GE Healthcare) equilibrated with the storage buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... desalted on a PD-10 column (GE Healthcare) equilibrated with 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2022Quote: ... After column mediated desalting (PD-10, GE Healthcare), fractions were exchanged to a buffer containing 10 mM Tricine (pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... A PD MiniTrap G-25 column (GE Healthcare) was used to remove imidazole ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Biophysics 2021Quote: ... Using a PD-10 desalting column (GE healthcare) buffer was exchanged to storage buffer (50 mM Tris pH 8.3 ...
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bioRxiv - Biophysics 2020Quote: ... using a PD-10 desalting column (GE Healthcare). Protein after PD-10 was labeled overnight at 4°C by immediately mixing with a 10-fold molar excess of the spin label (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol using PD-10 columns (GE healthcare). If required YpfP was concentrated using a Vivaspin 20 30MWCO until 2.4 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... using a PD-10 desalting column (GE Healthcare). Purified Ndc80C was stored at −80°C.
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bioRxiv - Cell Biology 2021Quote: ... and PD-10 (GE Healthcare, 17-0851-01) desalting columns ...
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bioRxiv - Biochemistry 2023Quote: ... and PD-10 columns (GE Healthcare, Uppsala, Sweden) were used for buffer exchange with storage buffer 20 mM HEPES pH 7.5 and 50 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and desalting (PD 10 Desalting Columns, GE Healthcare), the purified VitR protein was resolved by 15% SDS-PAGE.
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Biophysics 2021Quote: ... 1□mM DTT and 10% glycerol) using a PD MiniTrap G-25 sephadex columns (GE Healthcare) and the purified proteins were flash frozen in liquid N2 and stored at −80□°C ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
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bioRxiv - Biochemistry 2020Quote: ... and Sharpin were eluted and further purified over a Superdex 75 16/600 pd or a Superdex 200 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... Desalting was conducted using PD-10 column (GE healthcare) following manufacture’s protocol.
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bioRxiv - Plant Biology 2019Quote: ... pH 7.9 by PD-10 Desalting Columns (GE Healthcare). The 6xHis-Tag was removed by overnight digestion with thrombin protease ...
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bioRxiv - Biochemistry 2019Quote: ... using a PD-10 desalting column (GE Life Sciences). Protein was loaded onto a MonoQ anion exchange column (GE Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) using PD-10 desalting columns (GE Healthcare). Buffer exchanged protein was stored in aliquots at -80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were applied to PD-10 columns (GE Healthcare) to remove residual Zn2+ ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4) using PD-10 desalting columns (GE Healthcare), and stored at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... purified using a PD 10 desalting column (GE Healthcare) to remove excess PSMA and stored in pH 9 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 desalting column (GE Healthcare, Chicago, IL) was used to remove excess imidazole and buffer exchange in between the Ni affinity purification and thrombin cleavage ...
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bioRxiv - Biophysics 2023Quote: ... 0.1% DDM using PD-10 Desalting column (GE Healthcare). The desalted protein was immediately mixed with 40-fold excess of the spin label ...
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bioRxiv - Neuroscience 2023Quote: ... then loaded on a PD-10 column (GE healthcare). The columns were washed with buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...