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Citations for GE Life Sciences :
1 - 50 of 121 citations for Nucleic Acid Labeling since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Neuroscience 2019Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Microbiology 2023Quote: ... Radioactively labelled proteins or nucleic acids were visualised using storage phosphor screens (GE Healthcare) and a Typhoon 9400 imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid (400 ng) was spotted on to Amersham Hybond N+ nylon membrane (GE Healthcare Lifesciences), air-dried and UV cross-linked for 3 min using a transilluminator ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Cell Biology 2022Quote: ... URA3: CaUra3pXhoI and CaURA3m81) and Amersham ECL Direct Nucleic Acid Labelling and Detection System kit (GE Healthcare UK Limited ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Bioengineering 2019Quote: ... The nucleic acids were visualized using a G:BOX Chemi imager (Syngene, USA) and quantitative analyzed by ImageQuant (GE Healthcare, USA).
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bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Probe labeling for detection was performed using AlkPhos Direct Labeling and Detection System (GE Healthcare) according to the manufacturer’s description ...
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bioRxiv - Plant Biology 2021Quote: ... followed by [α-32P]dCTP-labeling by random priming (Multiprime DNA labeling kit; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... following labeling with Cy3 dye (GE Healthcare). Following incubation in the dark at 27°C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling with Cy5-NHS ester (GE Healthcare, PA15101) yielded 54–70% labeling efficiency ...
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bioRxiv - Plant Biology 2021Quote: ... Amersham Alkphos Direct labeling reagents (GE Healthcare, Buckinghamshire, UK) were used for probe labeling and subsequent hybridization ...
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bioRxiv - Microbiology 2021Quote: ... The probe was produced with a Rediprime II labeling kit (GE Healthcare). Hybridization was performed as described by Dienst et al ...
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bioRxiv - Microbiology 2019Quote: ... PCR fragments were then labelled using Megaprime DNA Labeling Systems (GE Healthcare). Primers used are listed in Table 1.
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bioRxiv - Microbiology 2020Quote: ... using the Amersham ECL Direct Labeling and Detection System® (GE Healthcare). Fungal RNA was isolated using TRIzol reagent (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The excess labeling agent was eliminated by using Hitarp desalting column (GE Healthcare). The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex) ...
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bioRxiv - Biochemistry 2020Quote: ... Confirmation of protein labeling was verified using a Typhoon imaging system (GE Healthcare). The labeled protein was aliquoted ...
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bioRxiv - Bioengineering 2024Quote: ... Radioactive probes were prepared by random-primed 32P labeling (GE Healthcare, Chicago, IL) of gel-purified DNA fragments of the following genes ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling was performed using an enhanced chemiluminescence (ECL) select kit (GE Healthcare Bio-Sciences). The chemiluminescence signals were detected in an ImageQuant LAS4000 mini (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2020Quote: ... radioactively labelled with α32P[CTP] using the Megaprime DNA Labeling System (GE Healthcare Life Sciences), and hybridized at 65 °C.
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bioRxiv - Plant Biology 2020Quote: ... α [32P] dCTP-labeled probes were generated by random priming (Megaprime DNA labeling kit; GE Healthcare). Hybridization was performed in Church Buffer (1% [w/v] BSA ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Amersham Rediprime II Random Prime Labeling System (RPN 1633; GE Healthcare, Chalfont St Giles, UK). Membranes were hybridized (250 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling of Spt7 and residual dye depletion were confirmed by in-gel fluorescence imaging on a Typhoon imager (GE Healthcare). In vitro transcription activity of the nuclear extract was verified by the in vitro transcription assay described below.
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bioRxiv - Immunology 2021Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare). T cells were activated for 2 days with anti-CD3/CD28 magnetic dynabeads (Thermo Fisher ...
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bioRxiv - Pathology 2019Quote: ... 5ug/mL Polyadenylic acid (GE Healthcare 27-4110-01), 2.5ug/mL pd (t)12-18 (Oligo dT ...
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bioRxiv - Pathology 2023Quote: ... packed with Ni2+ nitrilotriacetic acid (NTA)-sepharose (GE Healthcare) were used ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 32P-labeled DNA probes containing gag sequences were made by randomly primed DNA synthesis with Amersham Megaprime DNA labeling System (GE Healthcare). gag sequences were amplified by PCR from plasmids pGTy1-H3 (Genbank ...
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bioRxiv - Neuroscience 2020Quote: ... Northern blot probes to detect different isoforms of homothorax were prepared by Amersham Megaprime DNA Labeling System (GE Healthcare Life Sciences), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... A radioactively labeled probe corresponding to the coding region of TbGPI2 was generated using a Megaprime DNA-labeling system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% dextran sulfate) at 65°C probed with 32P-labeled CLB3 DNA probes prepared via Amersham Megaprime DNA labeling kit (GE Healthcare) and Illustra ProbeQuant columns (GE Healthcare) ...
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bioRxiv - Physiology 2020Quote: ... non-essential amino acids (GE Healthcare HyClone Laboratories, Logan, UT), 1X Penicillin/Streptomycin and Gentamicin (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Biochemistry 2019Quote: ... Labeling was confirmed by SDS-PAGE and then scanning the gel for fluorescence using a Typhoon FLA 9500 (GE Healthcare Life Sciences). Degree of labeling (DOL ...