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Citations for GE Life Sciences :
1 - 50 of 4028 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... Post-nuclear supernatants were incubated with 3 μg Protein A Sepharose CL-4B (PAS; Ge HealthCare, #GEH17078001) for 1 hour and then with 3 μg PAS plus primary antibody (0.4 ug anti-GFP mAb ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and resuspended them in Protein Extraction Buffer-V (GE Healthcare, Uppsala, Sweden) (urea ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Developmental Biology 2019Quote: ... nuclear extracts were first fractionated on High Trap Q (GE Healthcare) prior to Flag-IP ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclear/cytoplasmic distribution of YFP-FoxO3 was analysed using SoftWoRx (GE Healthcare) software.
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear and cytoplasmic extracts were subjected to Superose 6 size exclusion chromatography (GE Healthcare) with size exclusion buffer (40 mM HEPES [PH 7.5] ...
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bioRxiv - Molecular Biology 2023Quote: ... A PhytoPure DNA Extraction kit (GE Healthcare) was used to complete the DNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... These diluted nuclear extracts were pre-cleared using 25µL of previously blocked Streptavidin Sepharose beads (GE Healthcare) for 2h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... or lysed for RNA extraction (GE Healthcare, Buckinghamshire, UK). LT and mutant LT (E211K ...
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bioRxiv - Microbiology 2019Quote: ... using ‘Illustra DNA extraction kit Phytopure’ (GE Healthcare, Little Chalfont, UK). To ensure that DNA originated from endophytic microorganisms ...
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bioRxiv - Molecular Biology 2021Quote: ... The intensities of the bands of SREBP-1c and Lamin A/C in nuclear extracts were quantified with Image analysis software (ImageQuant TL, GE Healthcare). The intensities of nuclear SREBP-1c were normalized by those of corresponding Lamin A/C intensity.
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bioRxiv - Cell Biology 2023Quote: ... Images were deconvolved using an enhanced-ratio iterative constrained algorithm. Co-immunostaining images of nuclear bodies (Fig. 3c, Fig. 7c, Fig. 8d) were collected on OMX-V4 microscope (GE Healthcare) equipped with a U Plan S-Apo 100x/1.40-NA oil-immersion objective (Olympus ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted using the QuickPrep RNA extraction kit (GE HealthCare). Reverse transcribed using the TaqMan Reverse Transcription Reagent (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: RNA extraction was performed using illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted using Illustra RNAspin Mini RNA extraction kit (GE Healthcare, Buckinghamshire, UK). Three biological replicates per treatment (untreated and LT treated ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
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bioRxiv - Microbiology 2021Quote: ... and total RNA extraction was performed using Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). P ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Biochemistry 2020Quote: ... DNA was purified by phenol:chloroform extraction and centrifugation through a G-25 spin column (GE Healthcare). Samples were analyzed by agarose gel-electrophoresis and post-run ethidium-bromide stain.
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bioRxiv - Biochemistry 2020Quote: ... DNA was isolated by phenol:chloroform extraction and centrifugation through a G-25 spin column (GE Healthcare). Samples were fractionated on 0.8 % denaturing agarose gel in 30 mM NaOH / 2 mM EDTA ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted using gSYNC DNA Extraction kit (Geneaid) and quantified by nanovue (GE Healthcare). A touchdown PCR was performed on 200 ng of extracted genomic DNA with primers P488 and P650 to amplify a 759 bp fragment of ATP7B containing exon 6 and surrounding intronic regions ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Molecular Biology 2020Quote: ... coupled to protein G- and/or protein A-sepharose beads (GE Healthcare UK Ltd.). Proteins were eluted from beads with 0.2M glycine HCl pH2.5 ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...