Labshake search
Citations for GE Life Sciences :
1 - 50 of 2316 citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... fumigatus isolates were grown on 0.45 µm pore nylon membranes (GE Healthcare) placed on RPMI plates and incubated 1 day (without drug ...
-
bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
-
bioRxiv - Molecular Biology 2019Quote: ... the soluble lysate was loaded onto 1 mL Nickel beads (GE healthcare). The column was washed with lysis buffer containing 15 mM imidazole and his-m6A-Tracer was eluted by 250 mM imidazole in lysis buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were applied to tandem 1 mL HiTrap FF columns (Ge Healthcare) charged with Ni2+ ...
-
bioRxiv - Cell Biology 2020Quote: ... were added to isolate the epithelial cells using MagRack 6 (GE Healthcare 89129-096), and the rest cells in the suspension were mesenchymal cells ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 mg lysate was incubated with glutathione agarose resin (GE Healthcare, Chicago, USA) for 2 hours at 4°C under gentle rotation ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
-
bioRxiv - Microbiology 2019Quote: ... cell lysates were incubated with a DNase/ RNase mixture (1:100, GE Healthcare) for 40 min at 37°C and cell debris was subsequently removed by centrifugation (8,000 g ...
-
bioRxiv - Molecular Biology 2022Quote: ... The lysate was fractionated on a HisTrapTM HP 1 mL column (GE Healthcare) using a linear gradient from sonication buffer to elution buffer (sonication buffer with 250 mM imidazole) ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare) coupled to an ÄKTA Pure FPLC system (GE healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was applied to a 1 ml HisTrap HP column (GE Healthcare) and eluted with 300 mM imidazole in the same buffer without lysozyme ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysates were loaded onto a 1 ml HisTrap HP column (GE Healthcare) coupled to an ÄKTA Pure FPLC system (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2023Quote: ... lysates were shaken with a 1:1 mixture of hydrophilic and hydrophobic Sera-Mag SpeedBeads (GE Healthcare) in a final concentration of 50% ethanol for 8 mins at 23 ℃ ...
-
bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
-
bioRxiv - Biophysics 2021Quote: ... lysate was purified on a 1 ml Strep Trap HP (GE Healthcare, Chicago, IL) using the Äkta pure purification system (Cytiva ...
-
bioRxiv - Molecular Biology 2024Quote: Clarified lysate was passed over a 1-ml GSTrap Fast Flow Column (GE Healthcare) equilibrated with binding/wash buffer (20 mM phosphate pH 7.5 ...
-
bioRxiv - Systems Biology 2022Quote: ... The filtered lysate was loaded onto a 1 ml HisTrap FF (GE Healthcare, Freiburg, Germany) and unbound protein was removed with 20 column volumes of 15% buffer B (50 mM HEPES/KOH pH 7.8 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Ficoll-PaqueTM PLUS (17-1440-02) to isolate PBMCs from buffy coats was purchased from GE Healthcare.
-
bioRxiv - Molecular Biology 2021Quote: ... To isolate activated protein C (aPC) ion exchange chromatography with FPLC (ÄKTAFPLC®, GE Healthcare Life Sciences) was used ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2020Quote: ... The cleared lysates were loaded onto Ni-Sepharose beads and nutated for ∼1 h (GE Healthcare). Beads were washed twice with denaturing buffer and hexa-histidine-tagged proteins were eluted with the same buffer supplemented with 500 mM imidazole ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was incubated with 40 μl of glutathione-Sepharose beads (GE Healthcare) at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... lysed and the cell lysate was loaded onto a 1 ml GSTrap HP column (GE Healthcare) before elution using 50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The filtered cell lysate was loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
-
bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Reduced proteins were now purified from the lysate samples using SP3 magnetic beads (1:1 mixture of SpeedBead Magnetic Carboxylate Modified Particles 65152105050250 and 45152105050250, GE Healthcare). To enable protein binding to the beads ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
-
bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
-
bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were supplemented with 1 mM DTT and incubated with GSH-Sepharose 4B matrix (GE Healthcare) for 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... the lysate was loaded onto a 1 ml StrepTrap HP column (GE Healthcare Life Sciences, Buckinghamshire, UK) that was equilibrated with Lysis buffer.
-
bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
-
bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
-
bioRxiv - Synthetic Biology 2019Quote: The clean lysate was next loaded onto a HisTrap HP column (HisTrap FF crude 1 ml, GE Healthcare) at 4°C and flow rate of 0.5 ml min−1 ...
-
bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
-
bioRxiv - Biochemistry 2020Quote: ... lysates were loaded into a ResourceS (GE Healthcare) cartridge and eluted in a gradient of 0 to 500 mM NaCl in 50 mM ammonium acetate ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with lgG sepharose (GE Healthcare) at 4 °C for 2 hrs ...
-
bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with glutathione sepharose beads (GE Healthcare) and incubated overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Cleared lysates were incubated with IgG-Sepharose (GE Healthcare) at 4 °C for 2 h and eluted by TEV protease (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... lysates were incubated with streptavidin sepharose beads (GE Healthcare) overnight while rotating at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
-
bioRxiv - Biophysics 2019Quote: ... Lysate was loaded on to HisTrap HP (GE Healthcare), His-RanQ69L was eluted in binding buffer with 500mM Imidazole ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were loaded onto HisTrap crude column (GE Healthcare) on FPLC system (AKTA pure) ...
-
bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...