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Citations for GE Life Sciences :
1 - 50 of 6453 citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Microbiology 2022Quote: ... expressed proteins were detected using ECL chemiluminescent substrate (GE Healthcare, USA) in Biorad ChemiDoc MP™ Imaging System ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: The expressed protein was initially purified in an ÄKTA Start system (GE Healthcare) by immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Immunology 2020Quote: ... Expressed protein in supernatants were purified over a HisTrap excel column (GE Healthcare) and eluted in 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Glutathione Sepharose 4B beads (GE Healthcare), and then further purified with a HiLoad 16/600 Superdex 200 column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Neuroscience 2020Quote: GST-SH3-GK domain constructs of PSD-95 and MPP2 were expressed in E.coli BL21 DE3 and purified according to the manufacturer’s manual (GST Gene Fusion System, GE Healthcare). 30 µl of glutathione agarose (Pierce ...
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bioRxiv - Immunology 2021Quote: ... Expressed protein was isolated by metal affinity chromatography on HisTrap Excel columns (GE Healthcare), followed by GraFix methods using a 10% to 30% glycerol gradient and 0 to 0.1% glutaraldehyde gradient (Stark ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Microbiology 2020Quote: ... Expressed S2Pecto protein was isolated by metal affinity chromatography on HisTrap Excel columns (GE Healthcare), followed by further purification on a StrepTrap HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Ni Sepharose 6 Fast Flow beads (GE Healthcare). After removal of the His6-SUMO tag using home-made Ulp1 (SUMO protease) ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged expressed proteins were purified using GST beads (Glutathione Sepharose 4B; GE Healthcare Life Science). On the following day ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Cell Biology 2019Quote: ... The CiEB1-mCherry proteins expressed with histidine tag were purified using a Ni-NTA column (GE Healthcare) and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: RNA was isolated from F4/80+ macrophages using Illustra RNA Spin Mini kit (GE Healthcare). cDNA was made with High Capacity cDNA kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs used for protein expression and purification in this study were GST fusions expressed from pGEX-4T1 (GE Life Sciences). The native proteins without signal peptides were expressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2021Quote: ... Rosenfeld et al., 2021), expressed using ExpiCHO™ Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgG-YTE) were expressed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) and purified on HiTrap Protein-A column (GE healthcare, UK).
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bioRxiv - Cell Biology 2020Quote: Coding sequences were amplified from plasmids encoding mouse GPR161 (BC028163; Mammalian Gene Collection [MGC]; GE Healthcare), mouse SSTR3 (gift from Kirk Mykytyn ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-BrdU (1:1; GE Healthcare, RPN202); mouse anti-PCNA (1:500 ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Microbiology 2020Quote: ... The nsp7 and nsp8 genes were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Sequence verified human (clone IMAGE:5309813) and mouse (clone IMAGE:30060666) cDNAs for PDGFRβ were obtained from the Dharmacon Mammalian Gene Collection (GE Healthcare) for creation of surface-expressed GFP fusion receptor constructs ...