Labshake search
Citations for GE Life Sciences :
1 - 50 of 1632 citations for Mouse LIM Domain Kinase 2 LIMK2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
-
bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
-
bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
-
bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
-
bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
-
bioRxiv - Biophysics 2019Quote: ... After cleavage of the 6xHis-lipoyl domain with thrombin (GE Healthcare), the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was purified using a HisTrap excel column (GE Healthcare). The filtrated media were loaded into the HisTrap excel column ...
-
bioRxiv - Biochemistry 2023Quote: ... for the PWWP domain or a HiTrap Q HP column (GE Healthcare) for the ADD domain ...
-
bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The purified proteins or domains were immobilized on CM5 chip (GE Healthcare, USA) at response unit 2914.6 ...
-
bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
-
bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ARM domain was loaded on a Superose 6 Increase 10/300 (GE Healthcare) alone (at 2 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... The His6-GIGYF2 GYF domain was eluted from a Ni-NTA column (GE Healthcare) using 250 mM imidazole and further purified using a S200 10/600 column (GE Healthcare ...
-
bioRxiv - Biophysics 2022Quote: ... The His6-α2I domain was purified using a Ni2+-charged HisTrap HP (GE Healthcare Life) and buffer exchanged to PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was loaded into an anion-exchange chromatography column (HiTrap-Q, GE healthcare), and HER2 domain IV was collected from flow through.
-
bioRxiv - Biochemistry 2021Quote: The Src SH2 domain superbinder was purified and immobilized on CNBr-activated Sepharose (GE Healthcare) in IAP buffer (50 mM MOPS-NaOH [pH7.2] ...
-
bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
-
bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
-
bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
-
bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... DOCKDHR2 domains were further purified by a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare) and a RESOURCE™ Q column (GE Healthcare) ...
-
bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
-
bioRxiv - Microbiology 2023Quote: ... The SEALGGG domain was purified by size exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-KOH pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleaved START domain was purified by size exclusion chromatography on a HiLoad 16/60 column (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
-
bioRxiv - Genetics 2024Quote: ... the NHL domain was subjected to size-exclusion chromatography on HiLoad 16/600 Superdex 75 (GE Healthcare) to purify the NHL domain from GST and bacterial proteins ...
-
bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
-
bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
-
bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
-
bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...
-
bioRxiv - Biophysics 2019Quote: The purified Tom1 VHS domain was loaded onto an FPLC system using a Superdex 75 column (GE Healthcare) equilibrated with 50 mM Tris-HCl (pH 7.3) ...
-
bioRxiv - Cell Biology 2023Quote: ... the EfLigA domain of NMoRI was further purified by gel filtration using a Superdex-200 column (GE Healthcare) equilibrated against buffer (25 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... KIX domain was cleaved from GST by incubation of resin-bound GST-KIX with 160 U thrombin (GE Healthcare) overnight at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... VSG13 N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 50 mM HEPES ...
-
bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
-
bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
-
bioRxiv - Immunology 2023Quote: ... Contaminants were removed with 2-D Clean-up kit (GE Healthcare), and in-tube trypsin digestion was performed ...
-
bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
-
bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
-
bioRxiv - Microbiology 2020Quote: ... The VSGsur N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 10 mM Tris.Cl ...
-
bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
-
bioRxiv - Cell Biology 2021Quote: Purified His-tagged proteins anchor domain (2.1 mg/ml) was injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare), equilibrated with 20 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
-
bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
-
bioRxiv - Immunology 2021Quote: ... The head domain was purified from the supernatants by nickel affinity chromatography with HisTrap Excel columns (GE Healthcare Life Sciences), and H5.31 and H5.28 Fabs were purified with CaptureSelect IgG-CH1 (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 ml of purified WT SAM domain at 9.5 mg/ml was loaded onto a Superdex 75 16/60 gel filtration column (GE Healthcare) at 1 ml/min pre-equilibrated in buffer D ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...