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Citations for GE Life Sciences :
1 - 50 of 2612 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT). MCF7 cells used were authenticated to be 100% match to MCF7 cells (ATCC ...
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bioRxiv - Cancer Biology 2021Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT) at 37 °C in 5% CO2 ...
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bioRxiv - Pathology 2019Quote: ... 5ug/mL Polyadenylic acid (GE Healthcare 27-4110-01), 2.5ug/mL pd (t)12-18 (Oligo dT ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 U/ml penicillin and 100 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences). All cells were routinely cultured in a humidity incubator at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... using the 14N(p,α)11C route and converted to either [11C]MeI (methyl iodide) or [11C]MeOTf (methyl triflate) using a Tracerlab FX MeI module (GE Healthcare). A Tracerlab FX M module (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 U/mL penicillin/0.1 mg/mL streptomycin (GE Healthcare, USA), 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Neuroscience 2021Quote: ... and transferred onto a methanol-activated PVDF membrane (GE Healthcare) at 60 V for 12 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Developmental Biology 2022Quote: ... before being blotted onto methanol-activated 0.45μm PVDF membranes (GE Healthcare) at 300mA for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... 100 U/mL of Penicillin (GE Healthcare, catalog SV30010), and 100 µg/mL of Streptomycin (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... and 100 U/mL HyClone penicillin-streptomycin (GE Healthcare) at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
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bioRxiv - Immunology 2019Quote: ... and 100 µg/mL of Streptomycin (GE Healthcare, catalog SV30010)) at 37 °C/5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... and 100 mg/mL streptomycin (HyClone, GE Healthcare Life Sciences), at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 ml of 100 kU DNase I (GE healthcare) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... For StrepII IP 25 μl Streptactin Sepharose® High Performance beads (GE Healthcare, GE28-9355-99) and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension volume was adjusted to 33.4 mL with PBS and 10 mL of 100% isotonic Percoll (9 mL Percoll PLUS (GE Healthcare), 1 mL 10x PBS without Ca and Mg ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a methanol-activated Amersham Hybond PVDF membrane (GE Healthcare) at 100 V for 1 h at room temperature using Mini Trans-Blot Cell system (Biorad) ...
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bioRxiv - Cancer Biology 2023Quote: ... WCLs were incubated with 50 μl 7-methyl-GTP Sepharose 4B beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Patients were injected with 100–120 mL iohexol (Omnipaque, GE Healthcare) at an injection rate of 4–5 mL/s ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with 1.2 ml of fetal bovine serum (FBS) and 3.6 ml of 100% Percoll (GE Healthcare). The Percoll cell suspension was overlaid with 1 ml of 10% FBS in DMEM and spun at 800g for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Immunology 2020Quote: ... and penicillin (100 U/mL) (RPMI++) were transduced with filtered (0.45 μm) and concentrated (100 kDa molecular weight cutoff, GE Healthcare) HEK 293T supernatant ...
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bioRxiv - Synthetic Biology 2019Quote: ... and filtered before the cell extract sample was run through a nickel column (GE Healthcare, 11-0033-99). A low-concentration imidazole buffer was run through the column to wash out any non-specific proteins ...
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bioRxiv - Synthetic Biology 2020Quote: ... resuspended in methanol and loaded onto a Sephadex LH-20 (GE Healthcare, Little Chalfont, UK) column for fractionation ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred to previously hydrated with methanol PVDF membranes (Amersham™ Hybond, GE Healthcare) in a vertical tank (Mini-PROTEAN® Tetra Cell ...
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bioRxiv - Microbiology 2020Quote: ... gels were subjected to wet electrotransfer onto methanol-activated polyvinylidene difluoride (PVDF; GE Healthcare, Poland) membranes in 25 mM TRIS ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred to previously hydrated with methanol PVDF membranes (Amersham™ Hybond, GE Healthcare) in a vertical tank (Mini-PROTEAN® Tetra Cell ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils (purity > 97%, viability > 98%) were isolated from healthy heparinised peripheral blood using HetaSep (Stem Cell) and Ficoll-Paque (GE Healthcare), and contaminating erythrocytes were removed by hypotonic lysis as described previously (Wright et al. ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were electroblotted onto methanol-activated Amersham Hybond P0.45 polyvinylidene difluoride (PVDF) membranes (GE Healthcare) in Tris/Glycine transfer buffer (National diagnostics) ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatants obtained from the previous step were incubated with equilibriated Strep-Tactin Sepharose beads (28-9355-99, GE Healthcare) for 45 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was cleaved off by incubating the washed beads in wash buffer supplemented with PreScission Protease (∼100 mL at 76 mg/mL) (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates 30-50 ug were run on SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). Western blots were conducted using anti-CTLA-4/CD152 (LS C193047 ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was neutralized with 2 M Tris pH7.0 and either diluted first 1:1 with 20 mM phosphate pH7.0 buffer (25 mL milk sample) or loaded directly (100 mL milk sample) onto a Q Sepharose FF (GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM phosphate pH7.2 buffer column at 150 cm/h and 90 cm/h ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...