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Citations for GE Life Sciences :
1 - 50 of 320 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... MERS-CoV S1 was purified over a Superdex200 Increase column (GE Life Sciences). SARS-CoV-2 S-2P ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Neuroscience 2020Quote: ... as described and the radioiodinated S1 (I-S1) purified on a column of G10 Sephadex (GE Healthcare, Uppsala, SE), eluting with phosphate buffer (PB ...
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bioRxiv - Microbiology 2020Quote: ... or 10% FCS fetal clonal serum (FCS)(HyClone, GE Life Sciences), 100 U/ml penicillin and streptomycin (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... S1 and S2 were obtained using a DeltaVision platform (Applied Precision) composed of a customized inverted microscope (IX-71 ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... FCS (SH30071.03) from GE Healthcare Life Sciences and Puromycin from SIGMA (P9620) ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV NTD and SARS-CoV-1 NTD were then further purified using a Superdex 75 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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Persistence of learning-induced synapses depends on neurotrophic-priming of glucocorticoid receptorsbioRxiv - Neuroscience 2019Quote: ... Immunoreactivities (antibodies listed in Table S1) were revealed by chemiluminescence (GE Healthcare) and densitometric analysis of grayscale digital images using Chemidoc Touch (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS (GE Healthcare) and antibiotics (100 U ml−1 penicillin and 100 µg ml−1 streptomycin) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Spikes were further purified by Superose 6 increase 10/300 (GE healthcare) size-exclusion column in a buffer containing 2mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The Spike protein was purified on an Akta Express system (GE Healthcare) using a 5mL His trap FF GE Healthcare column in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... for the spike protein and a Superdex 75 10/300 (GE Healthcare) for the RBD and the RBD triple mutant showed a monodispersed and single peak with the expected molecular mass (Supplementary Figure 1).
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Immunology 2021Quote: ... 10% (v/v) FCS (Hyclone, GE Healthcare) and 0.1 mg/ml penicillin/streptomycin (15140122 ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... while the D614G VFLIP spike was purified on a StrepTrap XT column (GE Healthcare), according to the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... Fc was removed via ProteinA Sepharose incubation (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% fetal calf serum (FCS, GE Healthcare Life Sciences) and 500 U/mL penicillin-streptomycin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare), 100 µg/mL of streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% ultra-low IgG FCS (GE Healthcare), 100 μg/mL of Gentamycin ...
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bioRxiv - Bioengineering 2024Quote: ... Fc-fusion proteins in HBS-EP+ buffer (GE Healthcare) were immobilized at a capture level of 600-800 response units (RU ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were further purified on a Superose 6 gel filtration column (GE Healthcare) in 25 mM Tris pH 7.5 + 150 mM NaCl using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... HepG2 and HEK293 cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: Purified antibodies targeting S1 were quantified with SPR assay using the BIAcore 8000 system (GE Healthcare, USA) carried out at 25°C in single-cycle mode ...
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bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The 6xHis-Lipo tag was cleaved off using Thrombin (GE Healthcare) and the protein was separated from the cleaved tag using the same column and buffers as described above ...
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bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Cell Biology 2019Quote: ... followed by Step-tag affinity chromatography using StepTactin resin (GE Healthcare) in BRB20 ...
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bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS; Hyclone, GE Healthcare), 50 μM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...