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Citations for GE Life Sciences :
1 - 50 of 179 citations for Kinase Domain of Pyruvate Kinase liver Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... [γ-32P] (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Fischer) and subsequently purified using Illustra Microspin G25 columns (GE healthcare). Recombinant EB1 (0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Molecular Biology 2023Quote: ... The amount of [33Pγ]-tracer on the paper (signifying the kinase activity) was analyzed using a Typhoon FLA 700 IP PhosphorImager (GE Healthcare, Denmark), and related to the specific activity of the kinase reaction buffer ...
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Immunology 2022Quote: Liver macrophages were isolated by perfusing liver with 50%/25% Percoll (GE Healthcare, Pittsburgh, PA). Single-cell suspension of macrophages were incubated with Fc-blocker (2.4 G2 ...
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bioRxiv - Immunology 2022Quote: ... lymphocytes from livers were enriched using Percoll (GE Healthcare) density gradient separation (80%/40%) ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Biophysics 2019Quote: ... After cleavage of the 6xHis-lipoyl domain with thrombin (GE Healthcare), the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was purified using a HisTrap excel column (GE Healthcare). The filtrated media were loaded into the HisTrap excel column ...
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bioRxiv - Biochemistry 2023Quote: ... for the PWWP domain or a HiTrap Q HP column (GE Healthcare) for the ADD domain ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl/minute ...
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Immunology 2019Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Molecular Biology 2022Quote: ... Eluted peptides were fractionated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins or domains were immobilized on CM5 chip (GE Healthcare, USA) at response unit 2914.6 ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Leukocytes were isolated from livers using a 33.75% Percoll gradient (GE Healthcare). Following isolation ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ fetal liver cells were viably stored in 90% FBS (GE Healthcare) and 10% DMSO (FisherScientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The ARM domain was loaded on a Superose 6 Increase 10/300 (GE Healthcare) alone (at 2 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-GIGYF2 GYF domain was eluted from a Ni-NTA column (GE Healthcare) using 250 mM imidazole and further purified using a S200 10/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: EDC-crosslinked FERRY peptides were separated on a Superdex Peptide PC3.2/30 column (GE Healthcare) at a flow rate of 50 µL min−1 ...
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bioRxiv - Cancer Biology 2022Quote: Size based fractionation of the peptides were done using Superdex Peptide 3.2/300 (GE Healthcare) column and HPLC (Agilent 1260 Infinity II) ...
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bioRxiv - Immunology 2023Quote: Immune cells were isolated from livers by Percoll (17-08-91, GE Healthcare) separation ...
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bioRxiv - Physiology 2019Quote: ... supplemented with 1 mM sodium pyruvate (GE Healthcare, Thermo-Fisher), 1% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... The His6-α2I domain was purified using a Ni2+-charged HisTrap HP (GE Healthcare Life) and buffer exchanged to PBS ...
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bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was loaded into an anion-exchange chromatography column (HiTrap-Q, GE healthcare), and HER2 domain IV was collected from flow through.
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bioRxiv - Biochemistry 2021Quote: The Src SH2 domain superbinder was purified and immobilized on CNBr-activated Sepharose (GE Healthcare) in IAP buffer (50 mM MOPS-NaOH [pH7.2] ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography on a Superdex Peptide PC 3.2/30 column (GE Healthcare) using water/acetonitrile/TFA (77.4/22.5/0.1 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... hamsters were euthanised and amastigotes were purified from the liver by Percol gradient (GE Healthcare) after homogenisation ...
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bioRxiv - Biophysics 2021Quote: ... Peptides were desalted on a C18 MacroTrap column (Michrom Bioresources) and fractionated on a Superdex Peptide (GE Life Sciences) size-exclusion column ...
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bioRxiv - Biochemistry 2019Quote: ... 50 μg of peptides were fractionated in a Shimadzu HPLC system using a Superdex Peptide 3.2/300 (GE Healthcare) at a flow rate of 50 μl/min using SEC buffer (30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... DOCKDHR2 domains were further purified by a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare) and a RESOURCE™ Q column (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Microbiology 2023Quote: ... The SEALGGG domain was purified by size exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Cleaved START domain was purified by size exclusion chromatography on a HiLoad 16/60 column (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... the NHL domain was subjected to size-exclusion chromatography on HiLoad 16/600 Superdex 75 (GE Healthcare) to purify the NHL domain from GST and bacterial proteins ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...