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Citations for GE Life Sciences :
1 - 50 of 674 citations for Junctional adhesion molecule B JAM B Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Biochemistry 2021Quote: ... using buffer B (GE Healthcare Biosciences) and a flow rate of 1 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... YrbE1A/B-MceG and YrbE1A/B-MceGK43A were further purified and analyzed by SEC (AKTA, GE Healthcare) at 4 ⁰C on a pre-packed Superdex Increase 200 10/300 GL column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... 25 µg/mL amphotericin B (GE Healthcare) and maintained at 26°C in non-vented ...
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bioRxiv - Biophysics 2020Quote: ... 25 µg/mL amphotericin B (GE Healthcare) and maintained at 26°C in non-vented ...
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bioRxiv - Systems Biology 2023Quote: ... Sera-Mag Speed Beads A and B (Ge Healthcare) were vortexed until the pellet was dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... ActiCHO Feed A and ActiCHO Feed B (both GE Healthcare) started on day four based on a glucose-controlled feeding regimen ...
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bioRxiv - Biochemistry 2023Quote: ... whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare). Affinity tags were cleaved by incubation with either thrombin (Merck ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare) via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Antigen B was further purified from Fc using rProteinA Sepharose (GE Healthcare), followed by deglycosylation with endoH (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 mM b-ME) and purified over a Superdex 200 column (GE Healthcare). Octamer-containing fractions were pooled according to purity and histone stoichiometry ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) and a multi-frequency linear-array transducer (L4-12T ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: B cells were purified from spleens using Ficoll® Paque Plus (GE Healthcare) gradient centrifugation and negative depletion with FITC-conjugated mAb specific for CD4 (GK1.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) was used to determine the thickness of the VL of the right leg as previously described by our laboratory (18 ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Neuroscience 2023Quote: ... and were purified b y a ffinity ch romatography (GE Healthcare, Chicago, USA). Viral titer was determined by quantitative PCR using Taq-Man technology (Life Technologies ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Microbiology 2021Quote: Further purification of gyrase A and gyrase B used a mono-Q (GE Healthcare) column in buffer D and a linear gradient elution from 0 to 1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Addition of nutrient feeds ActiCHO Feed A and ActiCHO Feed B (both GE Healthcare) started when glucose levels dropped below ≤ 4 g x L-1 and was continued daily thereafter ...
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bioRxiv - Immunology 2020Quote: PBMCs from Donors A and B were isolated by Ficoll-Paque (GE Healthcare, Pittsburgh, PA) gradient centrifugation and cryopreserved ...
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bioRxiv - Molecular Biology 2021Quote: ... and the respective feed media ActiCHO Feed a and ActiCHO Feed B (both GE Healthcare). Inoculation cultures were obtained by expansion of ConM and ConS lead clones from MCBs in production medium (ActiCHO P medium supplemented with 8 mM L-glutamine ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 300 mM NaCl (IMAC B buffer) and loaded onto a 5 mL HiTrap IMAC column (GE Healthcare) using an Äkta Start Chromatography system ...
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bioRxiv - Plant Biology 2021Quote: Purification of cardosin B was performed and monitored by chromatography using an AKTA system (GE Healthcare, USA). 20X concentrated protein extract (10 ml ...
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bioRxiv - Microbiology 2022Quote: ... 1mM b-ME) and further purified by Size Exclusion Chromatography (HiLoad 26/600 Superdex 200, GE Healthcare), pre-equilibrated with buffer E (20 mM HEPES pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μg of GST or GST-Aurora B or GST-ERK was added to GSH beads (GE Healthcare, USA) in binding buffer (50 mM Tris-cl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... B-mode ultrasonography of the chest was performed (18;19) using a Logiq e system (GE Healthcare, Milwaukee, WI) using R5.2.x software and a multifrequency transducer model 12L-RS at a working frequency of 10 MHz ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
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bioRxiv - Biochemistry 2020Quote: ... using a linear gradient of buffer B to increase the concentration of imidazole on an ÄKTA purifier system (GE Healthcare). The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Molecular Biology 2024Quote: Ultrasonography images of the vastus lateralis (VL) were acquired with a B-mode imaging device (LOGIQ S7 Expert, GE Healthcare) using a multi-frequency linear array transducer (3-12 MHz ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...