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Citations for GE Life Sciences :
1 - 50 of 4951 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: Calorimetric binding experiments of Tim23IMS and TIM chaperones were performed using a MicroCal ITC200 instrument (GE Healthcare). Sixteen successive 2.5 µl aliquots of 1.15 mM Tim23IMS were injected into a sample cell containing 55 µM TIM9·10 or TIM8·13 ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Biophysics 2022Quote: ... The different histone chaperone constructs were purified by gravity-flow chromatography using a Ni-NTA resin (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superdex 200 Increase 10/300 column (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% iron-enriched bovine calf serum (BCS; GE Healthcare HyClone, #SH30072.03) and 1% MEM Non-essential amino acids (MEM NEAA ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% iron-enriched bovine calf serum (BCS; GE Healthcare HyClone, SH30072.03) and 50 U/ml of human IL-2 IS (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... The protein was repurified on Co-NTA agarose followed by gel filtration chromatography on Superdex 200 (GE Healthcare) in buffer A to remove the protease ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... Excess iron ammonium citrate and lithium sulfide was removed by desalting using a NAP™ 25 column (GE Healthcare). Subsequently ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Immunology 2021Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare) as described previously (Sok et al. ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare). Concentrations of purified mAbs was determined using a NanoDrop One (ThermoFisher).
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Cell Biology 2020Quote: ... and Cy5 (GE Healthcare Co., Ltd) NHS esters were dissolved in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Unbound iron was separated from the total complex using PD midiTrap-G25 columns following manufacturer’s instructions (GE Healthcare Bio-Sciences).
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Neuroscience 2019Quote: ... antibody-coated protein G–Sepharose beads (GE Healthcare; 20 μl) were added to the mixture and incubated on a shaker overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Antibody–protein interactions were revealed using chemiluminesence (RPN2108, GE Healthcare), and relative protein expression was quantified using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Plasma antibodies were purified using Protein A Sepharose resin (GE Healthcare), eluted from the resin with 0.1 M glycine at pH 2.5 and buffer exchanged into 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... The undigested antibodies were removed with Protein A Sepharose (GE Healthcare) and the flow through was collected ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified via HiTrap Protein A HP columns (GE Healthcare) run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare) ...
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bioRxiv - Genetics 2020Quote: ... using native Msh5 antibody and Protein A Sepharose beads (GE Healthcare). Briefly ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... Mitochondrial network and nuclei number were then assessed using InCell Analyzer 2200 (GE Healthcare, Chicago, Illinois, USA). Images were acquired in 16 fields per well at 10x magnification (Nikon 10x/0.45 ...
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bioRxiv - Biochemistry 2022Quote: ... Radioactive labeled mitochondrial translation products were detected using Phosphor screens and Amersham Typhoon imaging system (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... Recombinant antibodies were purified from supernatants using protein-G beads (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The antibody was purified by protein G affinity column chromatography (GE Healthcare) (Supplementary Fig ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from culture supernatant with Protein G Sepharose (GE Healthcare). One mg anti-IL-4Rα or an isotype control (rat IgG2a anti-trinitrophenol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were revealed using the appropriate HRP-conjugated secondary antibodies (GE healthcare). Proteins were then identified using Immobilon Western (Millipore ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-chromatin complexes were immobilized on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2000 RU ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified by Protein A affinity chromatography column (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...