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Citations for GE Life Sciences :
1 - 50 of 4465 citations for Interleukin 10 Receptor Beta IL10RB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Physiology 2020Quote: ... Insulin Receptor siRNA was obtained by GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Cell Biology 2020Quote: ... beta-Pix/ArghGEF7 (J-009616-07, Dharmacon GE Healthcare) or untargetting control were used ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... pH 7.4 (cytokines using Superdex 75 and receptors using Superdex200, prep grade media, GE Healthcare). In addition ...
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bioRxiv - Cell Biology 2020Quote: Receptor was further purified using anion-exchanged chromatography using HiTrap Q HP column (GE Healthcare). Protein was diluted 1:10 with buffer A (20 mM MOPS pH=7.0 ...
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bioRxiv - Biophysics 2023Quote: ... The eluted receptor was desalted from imidazole using a size-exclusion PD10 column (GE Healthcare) equilibrated with the desalt buffer (25 mM HEPES pH=7.5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Next the receptor was purified by size exclusion chromatography on a Sephadex S200 column (GE Healthcare) in 20 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: Affinity of bNAbs for Fcγ Receptors was assessed by SPR experiments performed on a Biacore T200 (GE Healthcare). SPR experiments were performed on a T200 apparatus at 25°C in PBS containing 0,05 % P20 surfactant (Cytiva) ...
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bioRxiv - Cancer Biology 2019Quote: ... The clicked antibodies were purified by SEC (Superdex 200, 10×300 GL, GE Healthcare) at a flow rate of 0.5ml/min in PBS using a GE AKTAPurifier ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Microbiology 2019Quote: ... The labelled antibodies were purified by passing through Disposable PD 10 Desalting Columns (GE Healthcare Life Sciences), concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Microbiology 2022Quote: ... The antibodies were purified on an AKTA FPLC using GE Superdex 200 increase 10/300 GL column (GE HealthCare) in 1× PBS.
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bioRxiv - Cancer Biology 2022Quote: ... using HRP-conjugated secondary anti-mouse antibody (2.5:10 000) and Amersham ECL prime detection reagent (GE healthcare lifescience).
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bioRxiv - Immunology 2022Quote: Antibody Fab fragments were purified further via size-exclusion chromatography using an S200 increase 10/30 column (GE Healthcare) equilibrated with 25 mM Tris (pH 8.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... The unconjugated DFO was removed from the antibody-DFO conjugates using a size exclusion column (PD-10; GE Healthcare) and the antibody solutions were concentrated using amicon filters with a 50 kDa cutoff filter ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting elutes containing antibodies were applied to a size exclusion chromatography column (Superdex 200 10/300 GL; GE Healthcare) with PBS served as running buffer and a flow rate of 0.5 mL/min (SI Figure 8) ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol using PD-10 columns (GE healthcare). If required YpfP was concentrated using a Vivaspin 20 30MWCO until 2.4 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Mono Q 10/10 (GE Healthcare Life Sciences), and HiLoad 16/600 Superdex 200 pg (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) using PD-10 desalting columns (GE Healthcare). Buffer exchanged protein was stored in aliquots at -80°C.
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bioRxiv - Bioengineering 2021Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent: PBS, flow rate = 0.6 mL/min) to afford an antibody–linker conjugate [1.91 mg ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mM MgCl2) using a PD-10 desalting column (GE Healthcare) and aliquots were snap-frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10% glycerol) on a PD-10 column (GE Healthcare, 17085101), concentrated with a 10 kDa MWCO Amicon Ultra-4 device (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM Imidazole using a Desalting 26/10 column (GE Healthcare). The His-tag was then removed by incubation with the tobacco etch virus (TEV ...
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bioRxiv - Immunology 2021Quote: ... and the homogeneity and the size of the antibodies were analyzed by using an analytical Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biochemistry 2019Quote: ... and buffer exchanged to 10 mM phosphate buffer pH 7.4 containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.) ...
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bioRxiv - Biochemistry 2023Quote: ... Resin was poured into 10 mL empty PD-10 columns (GE Healthcare) and all washes and elution were performed by gravity flow with buffers as used for WT purification ...
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bioRxiv - Bioengineering 2020Quote: ... HyClone (10%; GE Healthcare) and penicillin/streptomycin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... HyClone (10%; GE Healthcare) and penicillin/streptomycin (1% ...
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bioRxiv - Biochemistry 2020Quote: ... PDIs and their mutants were separately reduced with 10 mM DTT for 10 min at 30°C followed by DTT removal through a PD-10 column (GE Healthcare) pre-equilibrated with 50 mM Tris/HCl (pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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bioRxiv - Neuroscience 2023Quote: ... at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000–4000 response units (RUs) ...