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Citations for GE Life Sciences :
1 - 35 of 35 citations for Influenza Virus Hemagglutinin HA H9N2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... lymphocytes from the lungs of influenza virus infected mice were enriched using Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient and stained with H-2Db restricted tetramers conjugated to fluorophore R-phycoerythrin (PE ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The cell line has undergone selection in serum-free insect cell medium (HyClone, GE Healthcare) containing 7 μg/ml puromycin and 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... Fractions corresponding to HA-Trimers were further polished via gel filtration using Superdex 200 (GE Healthcare) according to manufacturer’s instructions to change buffer and then concentrated by ultrafiltration into PBS.
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bioRxiv - Microbiology 2023Quote: ... Cleaved HA heads were then further purified by gel filtration chromatography on Superdex 200 (GE Healthcare) in buffer A.
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 45 µL of 42 µM Jagged1fe,HA were loaded on a Superose6 3.2/300 increase (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Analytical size exclusion chromatography has been performed with a Superdex 75 Increase 3.2/300 column (GE Life Sciences) and 20 mM sodium acetate ...
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bioRxiv - Immunology 2023Quote: ... HA trimers were purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare). Fractions corresponding to trimeric HA were pooled ...
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bioRxiv - Immunology 2023Quote: ... Recombinant HA molecules were purified from these culture supernatants by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with imidazole gradient ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Molecular Biology 2023Quote: ... was processed for amplification of complete genome of the virus by rolling circle amplification (RCA) method using TempliPhi illustra amplification kit (GE Healthcare, Piscataway, NJ). The resulting RCA product (2 μL ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of GDAP1Δ319-358 and GDAP1Δ295-358 towards HA was measured using a MicroCal iTC200 calorimeter (GE Healthcare) in SEC buffer with 2% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Cell Biology 2020Quote: ... the BglII–SalI fragment of HA-Catalase amplified by PCR from pUcD3/HA-HsCatalase (Otera and Fujiki, 2012) was cloned into the BamHI–SalI sites of pGEX6P-1 (GE Healthcare), thereby generating pGEX/HA-HsCatalase ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Biophysics 2020Quote: ... Immunoblots with anti-HA, anti-His or anti-SecB antibodies were visualized by Lumino image analyzer LAS-4000 mini (Cytiva, Tokyo, Japan (GE Healthcare)) using ECL Prime Western Blotting Detection Reagents (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... clarified lysates were mixed with 1 µg anti-HA monoclonal antibody and rotated with 30 µl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cell Biology 2019Quote: ... -S168D and -S168E fragments were cut from pcDNA3-HA-PI31 vectors using EcoRI and XhoI and inserted into the pGEX-4T1 vector (GE Healthcare, #28954549) to generate pGEX-4T1-PI31 clones ...
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bioRxiv - Immunology 2020Quote: ... and soluble HA trimers were purified from transfected cell supernatants by standard Ni-NTA chromatography using a prepacked HisTrap™ HP column (GE Healthcare) and SEC using a HiLoad® 16/600 Superdex® 200 column (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...