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Citations for GE Life Sciences :
1 - 50 of 1306 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Full-length Pak1 constructs (wild type and catalytically inactive) and full-length Sts5 constructs (wild type and phosphomutant) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare) for expression in BL21 DE3 Escherichia coli ...
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bioRxiv - Immunology 2019Quote: ... resuspended in 35% Percoll (GE Healthcare) and centrifuged ...
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bioRxiv - Biochemistry 2021Quote: ... The separation of unreacted [35S]-fMet and each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Microbiology 2021Quote: ... The separation of unreacted [35S]-fMet from each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Neuroscience 2022Quote: ... tissue was resuspended in 35% isotonic Percoll (GE Healthcare) in HBSS overlaid with 1X HBSS and centrifuged (800 x g ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was resuspended in 35% isotonic Percoll (GE Healthcare) overlaid with HBSS and centrifuged (800 x g ...
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bioRxiv - Molecular Biology 2019Quote: ... Protease cleavage (PreScission: GE Healthcare; TEV-Protease: Biomol) was performed overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and cells were enriched using a 35% Percoll (GE Healthcare) gradient.
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bioRxiv - Biochemistry 2022Quote: ... the peptides were passively eluted into 72 contiguous fractions with MilliQ water/ 35% acetonitrile (CAN)/ 35% ACN + 0.1% formic acid (FA) using an in-house constructed IPG extraction robot (GE Healthcare Bio-Sciences AB, prototype instrument) into a 96-well plate (V-bottom ...
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bioRxiv - Developmental Biology 2020Quote: ... of FZD7 containing the epitope for the F7-Ab (Glycine 168 to Serine 209) was cloned into the bacterial GST expression vector pGEX-4T3 (GE Healthcare Life Sciences). The epitope was shortened from either end through sequential cloning to produce 10 different length epitopes ranging from the original 42 aa to 8 aa ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was aspirated and pellets resuspended in 35 % Percoll (GE healthcare) in HBSS (without Ca2+ & Mg2+ ...
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bioRxiv - Immunology 2023Quote: ... leukocytes were purified by resuspending pellets in 35% Percoll (GE Healthcare) and HBSS followed by room temperature centrifugation at 1800 RPM ...
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bioRxiv - Synthetic Biology 2020Quote: ... and desalted on a Sephadex G25 XK 26/35 column (GE Healthcare). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Pellet was then resuspended in 35% percoll (GE Healthcare, #17-0891-01) and centrifuged for 20 minutes at 800 g at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... PreScission Protease (GE Healthcare) was added to remove GST from the proteins ...
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bioRxiv - Plant Biology 2020Quote: ... intact chloroplasts were re-purified by centrifugation through a 35% Percoll (GE Healthcare) solution in IB with 5 mM EDTA.
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bioRxiv - Biophysics 2020Quote: ... The GST was cleaved with GST-tagged HRV 3C protease (PreScission Protease, GE Healthcare). The GST and protease was removed with glutathione sepharose resin ...
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bioRxiv - Cancer Biology 2019Quote: ... After centrifugation the cell pellet was resuspended in a 35 % Percoll solution (170891, GE Healthcare) and a phase of 70 % Percoll was underlaid using a glass Pasteur pipette ...
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bioRxiv - Immunology 2019Quote: ... the mononuclear cells were isolated on a discontinuous 70% and 35% Percoll gradient (GE Healthcare) by centrifugation at 700g for 20 minutes without brake ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded into 35-mm dishes with 1.5-thickness coverglass bottom (Fluorodishes - Applied Precision) at ∼50,000 cells per dish and grown for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Images were acquired in 25-35 Z-slides and deconvolved using softWoRx software (GE Healthcare). Images were analyzed using Fiji (Image J ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin protease (2U/mg GE healthcare) was added ...
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bioRxiv - Cell Biology 2023Quote: ... complete protease inhibitor mix (GE Healthcare), 5 percent glycerol (v/v) ...
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bioRxiv - Molecular Biology 2023Quote: ... ‘PreScission Protease’ (GE Healthcare Life Sciences) was used according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... 35S-labelled proteins were monitored using autoradiography and visualised using a Typhoon 9400 scanner (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The isolated tumor infiltrating immunce cells were centrifuged in 35% v/v Percoll solution (GE Healthcare) and RPMI (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and then loaded onto a HiLoadTM 16/600 SuperdexTM 200 pg (GE Healthcare, 28-9893-35) gel filtration column pre-equilibrated with 50 mM HEPES pH 7.0 ...
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bioRxiv - Immunology 2023Quote: ... Hepatocytes were separated from immune cells by a 35% and 70% bilayer Percoll gradient (GE Healthcare) and used.
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bioRxiv - Biochemistry 2020Quote: ... once loaded GST-PreScission Protease (GE Healthcare) was injected into the column and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... by cleavage with PreScission protease (GE Healthcare) which cleaves between GST and Nuf2 in the GST-Nuf2 fusion protein ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty units of PreScission Protease (GE Healthcare) were added ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNA encoding mouse TRIM41 (residue 35-85 aa, NP_663352.2) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... layered (appr. 35 mL per tube) onto 15 mL Ficoll-Paque PLUS (GE Healthcare 17-1440-02) using SepMate-50 tubes (Stem Cell Technologies 85450) ...
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bioRxiv - Biochemistry 2019Quote: ... and then further purified via gel filtration (Superdex 200 HiLoad 16/600, GE Healthcare – 28-9893-35) in CSF-XB buffer (0.01M Hepes ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 35S signal collected on the phosphor screens was scanned using a Typhoon FLA-7000 scanner (GE Healthcare). Due to the poor accessibility of the substrate signal peptide in vitro ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35) with wash buffer ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Biophysics 2021Quote: SPR measurements were recorded at either 25°C or 35°C using a Biacore T200 system (GE Healthcare). All experiments were performed using an SA Series S Sensor Chip (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: 35S-positive bands within NP40 cell extracts were detected in a Typhoon system (GE Healthcare Life Sciences, UK). The autoradiography protein profile observed in the non-supplemented or Cu-supplemented cells was represented as a heatmap of blot intensity (ranging white to blue ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Biochemistry 2021Quote: ... Sample was incubated with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Immunology 2021Quote: ... After addition of 12μl PreScission Protease (GE Healthcare), the suspension was incubated under rotation for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... followed by digestion with PreScission protease (GE Healthcare) and further purification by RESOURCE Q 6 mL column chromatography (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitors were either from GE Healthcare (cOmplete) or Thermo Scientific (Halt− protease inhibitor cocktail) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged HRV 3C protease (produced in-house) was used instead of TEV protease and a HiLoad 16/600 S200pg SEC column (GE Healthcare) was used instead of a HiLoad 26/600 S200 SEC column ...
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography on gel filtration columns using either the Superdex 200 (16/600) (GE Healthcare, GE28-9893-35) or Superdex 75 (16/600 ...