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Citations for GE Life Sciences :
1 - 50 of 202 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Physiology 2020Quote: ... Insulin Receptor siRNA was obtained by GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... and 6,000 Ci/mmol γ-[32P] ATP (GE Healthcare). Reactions were quenched by the addition of Laemmli sample buffer and analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Supernatant from transfected CHO cells was purified using 1 ml HiTrap MabSelect PrismA (GE Healthcare – 17549851) affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684 ...
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bioRxiv - Molecular Biology 2023Quote: ... Unbound [γ-32P]-ATP was removed by MicroSpin G-25 columns (GE Healthcare). The RNA was separated by electrophoresis in a denaturing 18 % polyacrylamide gel ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant TSP2 protein was purified from CHO cells expressing mouse TSP2 via affinity chromatography with HiTRAP heparin HP (GE Healthcare) following protocols described by Oganesian et al ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... pH 7.4 (cytokines using Superdex 75 and receptors using Superdex200, prep grade media, GE Healthcare). In addition ...
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bioRxiv - Cell Biology 2020Quote: Receptor was further purified using anion-exchanged chromatography using HiTrap Q HP column (GE Healthcare). Protein was diluted 1:10 with buffer A (20 mM MOPS pH=7.0 ...
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bioRxiv - Biophysics 2023Quote: ... The eluted receptor was desalted from imidazole using a size-exclusion PD10 column (GE Healthcare) equilibrated with the desalt buffer (25 mM HEPES pH=7.5 ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Next the receptor was purified by size exclusion chromatography on a Sephadex S200 column (GE Healthcare) in 20 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... The MtaLonA-Ig2-MMH8709-ATP-γ-S complex was then loaded onto Superdex 200 (GE Healthcare) column pre-equilibrated with 20 mM Tris-HCl (pH8.0) ...
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bioRxiv - Microbiology 2024Quote: ... for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T, GE Healthcare, code NA934V); for EF1-α - anti-mouse (1:10,000 in 1% milk/PBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... A Multiphor II (GE Healthcare) was used for isoelectric focusing ...
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... After removal of unbound γ-ATP by MicroSpin G-25 column (GE Healthcare, Thermo Fisher Scientific Inc.) purification ...
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bioRxiv - Immunology 2024Quote: Affinity of bNAbs for Fcγ Receptors was assessed by SPR experiments performed on a Biacore T200 (GE Healthcare). SPR experiments were performed on a T200 apparatus at 25°C in PBS containing 0,05 % P20 surfactant (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Unincorporated γ-32P ATP was removed by running the product through Illustra MicroSpin™ G-25 Columns (GE Healthcare) and probe radioactivity was measured.
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bioRxiv - Microbiology 2023Quote: ... the labelled oligonucleotides were separated from free [γ-32P] ATP using a custom Sephadex G-25 column (GE Healthcare). Labelled nucleic acids were incubated with nucleases (Rnase A ...
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bioRxiv - Biochemistry 2021Quote: ... [γ-32P] (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Fischer) and subsequently purified using Illustra Microspin G25 columns (GE healthcare). Recombinant EB1 (0.5 ...
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Cell Biology 2021Quote: ... and the signal of γ-32P-ATP was detected using a PhosphorImager scanned by Typhoon 8600 Variable Mode Imager (GE Healthcare). Bands on the autoradiograms correspond to the molecular weight where Coomassie-stained protein migrates (Figure 1-figure supplement 1).
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bioRxiv - Physiology 2020Quote: ... and a Multiphor II Electrophoresis System (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were dried for one hour and the signal of γ-32P-ATP was detected via a PhosphorImager scanned by Typhoon 8600 Variable Mode Imager (GE Healthcare). For Fig ...
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bioRxiv - Microbiology 2021Quote: ... The labelled probes were then diluted in 40 µl of water and separated from free [γ-32P]dATP by gel filtration (Illustra Microspin columns; GE Healthcare). Dried membranes were pre-hybridized in 8 ml of pre-warmed ExpressHyb buffer (Clontech ...
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bioRxiv - Biochemistry 2021Quote: ... Subsequently the gel was washed several times with water to remove residual γ-32P-labelled ATP and phosphoproteins were detected using autoradiography and a Amersham Typhoon RGB biomolecular imager (GE Healthcare). Phosphoprotein signal (P-UPF1 or P-mTORΔN ...
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bioRxiv - Genomics 2021Quote: ... G-25 sephadex™ G-50 DNA grade resin columns were used to remove unincorporated γ-32P ATP according to manufacturer’s instructions (illustra™ GE Healthcare). The purified strand was used for subsequent annealing with equal amount of complementary strand in 50 μL total volume ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2021Quote: ... The samples loaded on SDS-PAGE and γ-32P phosphorylation signals were detected using Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Microbiology 2021Quote: ... Primers were labelled using T4 PNK in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol) and purified using G-50 Microspin columns (GE Healthcare, #27-5330-01). Reverse transcription reactions were stopped by adding 40 μL of cold 0.3 M Na-acetate ...
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bioRxiv - Biochemistry 2023Quote: ... Streptactin II agarose was from GE healthcare (Little Chalfont, UK). Myc-trap agarose was from Chromotek (Planegg ...