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Citations for GE Life Sciences :
1 - 50 of 595 citations for IDO Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Affinity of IDO-Gal3 for lactose was determined using affinity chromatography in an AKTA Pure chromatography system (GE Life Sciences) equipped with consumer-packable glass column (GE Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-Trap Q (GE Healthcare), and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... and His-trap column (GE healthcare) previously equilibrated with Lysis Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... using a His SpinTrap column (GE Healthcare). Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Microbiology 2023Quote: ... Anti-His antibody was from GE Healthcare; anti-PhoP antibody was elicited in rabbit (AlphaOmegaSciences) ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Both the cleaved His-tag and the His-tagged protease were removed from protein solutions by passage over loose nickel beads (GE Healthcare). The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and ion-exchange (Hi-Load Q column, GE Healthcare) for the 5FW-labeled protein ...
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bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Microbiology 2020Quote: ... The six-His-tagged BclA3CTD protein was purified under native conditions using the His-Trap column as recommended by the manufacturer (GE Healthcare Life Science). The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...