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Citations for GE Life Sciences :
1 - 50 of 4680 citations for Human Ring Finger Protein 7 RNF7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Microbiology 2019Quote: ... Supernatants were precleared using 20µl of protein G sepahrose beads (GE Healthcare, 7-0618-01) for 1hr at 4°C ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 7 cm strips (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 4 (excepting E) were performed at room temperature using the ring TIRF light path of a DeltaVision OMX SR microscope (GE Healthcare) with a 60x/1.42 numerical aperture (NA ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Microbiology 2021Quote: ... (7) Superdex 75 resin (GE Healthcare) chromatography using an isocratic elution in Tris buffer B and (8 ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Proteins were precipitated using the 2D Clean-up Kit (GE Healthcare). The pellet was reconstituted in 50mM ammonium bicarbonate and digested with Trypsin (1/50 w/w ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Immunoreactive proteins were visualized using an ECL prime kit (GE HealthCare).
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of proteins was performed using the ECL kit (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... SEC columns were calibrated with a protein standard kit (GE Healthcare) containing ferritin (MW = 440 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected with ECL Advance kit (GE Healthcare,Poole UK).
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Bioengineering 2022Quote: ... gels were either silver stained (PlusOne Silver Staining Kit, Protein; GE Healthcare) or transferred onto nitrocellulose membranes for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated using the 2D Clean Up Kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were detected using enhanced chemiluminescence (ECL) detection kit (GE HealthCare, RPN2235).
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bioRxiv - Microbiology 2019Quote: Protein concentration was measured with the “2D Quant kit” (GE Healthcare Life Sciences), according to the supplier’s instructions ...