Labshake search
Citations for GE Life Sciences :
1 - 50 of 5663 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: Plasma M- and Z-AAT was isolated by affinity chromatography using the AAT specific Alpha-1 Antitrypsin Select matrix (GE Healthcare Life Sciences, Cytiva, Sheffield, UK) according to the manufacturer’s recommendations ...
-
bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein expression was quantified using the Alpha Innotech software for the FluoroChemQ chemiluminescent exposure system (Alpha Innotech; GE Healthcare, London, ON, Canada) or ImageLab for ChemiDoc system (BioRad ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
-
bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
-
bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
-
bioRxiv - Bioengineering 2019Quote: Biacore T200 instrument and related reagents (GE Healthcare) were used to perform all Surface Plasmon Resonance experiments ...
-
bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
-
bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
-
bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
-
bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
-
bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
-
bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
-
bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
-
bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
-
bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
-
bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
-
bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
-
bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
-
bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
-
bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
-
bioRxiv - Molecular Biology 2020Quote: ... coupled to protein G- and/or protein A-sepharose beads (GE Healthcare UK Ltd.). Proteins were eluted from beads with 0.2M glycine HCl pH2.5 ...
-
bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
-
bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
-
bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
-
bioRxiv - Bioengineering 2023Quote: ... All fusion proteins were purified by using a protein A affinity column (GE Healthcare) and analyzed on SDS-PAGE in the reducing condition.
-
bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.