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Citations for GE Life Sciences :
1 - 50 of 4673 citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Physiology 2023Quote: ... Seramag speedbead carboxyl beads (GE Life Sciences) were prepared where a 1:1 mix of hydrophilic (Cat No ...
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bioRxiv - Cancer Biology 2022Quote: ... a bead slurry was prepared by mixing 50 μl of Sera-Mag SpeedBeads Carboxyl Magnetic Beads (hydrophobic) with 50 μl of Sera-Mag SpeedBeads Carboxyl Magnetic Beads (hydrophilic) (both from GE Healthcare). The bead slurry was washed two times with 200 μl of MQ water using a magnetic rack ...
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bioRxiv - Cell Biology 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Bioengineering 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...
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bioRxiv - Cell Biology 2021Quote: ... Hydrophilic and hydrophobic Cytiva Sera-Mag Carboxyl-Magnet-Beads (GE Healthcare) were mixed 1:1 and 2 μl beads (25 μg/μl ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was removed from unbound ligand by adsorption to Q-sepharose beads (GE healthcare, USA), the beads were washed ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Genomics 2022Quote: ... with 1.0x volume of 0.4% solution of SeraMag SpeedBeads® Carboxyl Magnetic Beads (GE Healthcare), followed by an incubation of 30-60 min ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Microbiology 2020Quote: ... The protein and ligand were incubated for 10 min and spotted onto Protran BA85 nitrocellulose (0.45 μm, GE Healthcare) with a replicator tool (VP 408FP6S2 ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... which contained two additional residues (Gly-Ser) upstream of the PDZ tandem, was further purified using cation-exchange chromatography HiTrap SP HP (Cytiva, Tokyo, Japan (GE Healthcare)) and size-exclusion chromatography Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Bioengineering 2020Quote: ... Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988) via amine coupling ...
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bioRxiv - Bioengineering 2023Quote: ... Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988) via amine coupling ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... Sample clean-up and library preparation was carried out using Sera-Mag Speedbeads hydrophobic carboxyl magnetic beads (GE Healthcare, 65152105050250). Following bead cleanup ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... TEV protease was added during dialysis to remove the N-terminal His6-tag.The protein was retrieved from dialysis and loaded onto a 1ml HiTrap SP HP IEX column (GE Healthcare) and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Systems Biology 2021Quote: ... The Ni-elution was cleaved with TEV protease and the PDZ-ANXA2 was purified by cation exchange on a HiTrap SP HP column (GE Healthcare, Chicago, Illinois). Proteins were aliquoted at 5-7 mg/ml concentration before flash freezing in LN2 ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... The magnetic bead mixes were prepared by combining equal parts of Sera-Mag Carboxyl hydrophilic and hydrophobic particles (09-981-121 and 09-981-123, GE Healthcare). The bead mix was washed three times with MS-grade water and resuspended in a working concentration of 10 µg/µl ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated Omicron RBD ligand was immobilised on a SA sensor chip (GE Healthcare) at 3622 RUs ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Biophysics 2019Quote: ... Labeled protein was separated from unreacted fluorophore and the Sfp synthase by size-exclusion chromatography on a superose 6 3.2/200 (GE Healthcare) preequilibrated in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated Wuhan RBD ligand was immobilised on a streptavidin-coated (SA) sensor chip (GE Healthcare) at 2453 RU ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Analyte to ligand binding experiments were done in the Biacore 3000 instrument (GE Healthcare Life Sciences) at 25°C ...
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bioRxiv - Plant Biology 2019Quote: ... Ligands were coupled to NHS-activated Sepharose™ 4 Fast Flow beads (GE Healthcare, United Kingdom). Control beads blocked with ethanolamine were prepared in the same way ...
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bioRxiv - Biochemistry 2023Quote: ... Excess HaloTag Ligand was removed by gel filtration on a PD-10 column (GE Life Sciences) and re-concentrated by ultrafiltration.
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...