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Citations for GE Life Sciences :
1 - 50 of 745 citations for HCC 4 CCL16 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2023Quote: ... covered in 4+4 Whatman papers (GE Healthcare, UK) soaked in transfer buffer (192 mM glycine ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Biochemistry 2019Quote: ... Following dialysis the E215C K490 construct was reacted for 4 h at 4°C with Cy3-maleimide (GE Healthcare, PA13131) at a motor/Cy3 dye ratio of 1:10 as previously described (Tomishige et al ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... and glutathione Sepharose 4 Fast Flow (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Molecular Biology 2021Quote: ... nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Biochemistry 2022Quote: ... and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) following the instructions provided by the manufacturer with appropriate modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μL were spotted onto dry nitrocellulose (GE Healthcare) in 15-second increments ...