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Citations for GE Life Sciences :
1 - 50 of 4462 citations for Glial fibrillary acidic protein GFAP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Both the cleaved His-tag and the His-tagged protease were removed from protein solutions by passage over loose nickel beads (GE Healthcare). The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Microbiology 2020Quote: ... The six-His-tagged BclA3CTD protein was purified under native conditions using the His-Trap column as recommended by the manufacturer (GE Healthcare Life Science). The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore) ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Plant Biology 2020Quote: ... tNPR1-His proteins were further purified by HiLoad 16/600 Superdex 200 (GE Healthcare) with 200 mM NaHCO3 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Immunology 2021Quote: ... RBD protein was then captured using His-Trap HP column (GE Healthcare, Wauwatosa, WI, USA) or HiTrap TALON crude column (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The His-tagged proteins were purified by cobalt affinity chromatography using Chelating Sepharose (GE Healthcare). The proteins were eluted in buffer containing 25 mM Hepes-KOH (pH 7.4) ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins in the supernatants were purified using HIStrap FF columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.