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Citations for GE Life Sciences :
1 - 50 of 127 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: HEK 293 cells were cultivated in DMEM (GE Healthcare), supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: HEK-293 were cultured in DMEM high-glucose (GE Healthcare cat# SH30081.01) containing 10% bovine calf serum (GE Healthcare cat# SH30072.03) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... 48 h later the culture volume was doubled by feeding HyClone SFM4Transfx-293 media (GE Healthcare) supplemented with 8 mM Glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... After 48 h the culture volume was doubled by feeding HyClone SFM4Transfx-293 media (GE Healthcare) supplemented with 8 mM Glutamine ...
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bioRxiv - Biochemistry 2019Quote: HEK-293T cells (ATCC catalog number CRL-3216; RRID:CVCL_0063) maintained in DMEM high glucose (GE Healthcare) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T were obtained from ATCC and cultured at 37°C and 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant antibodies were produced in a transient HEK expression system and purified via protein A affinity purification on an automated AKTA system (AKTA explorer FPLC, GE Healthcare). For in vivo studies rabbit V-regions were fused with murine constant of mouse IgG2a heavy chain and kappa chain to minimize immunogenicity ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...