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Citations for GE Life Sciences :
1 - 50 of 169 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Gels were stained with Coomassie Blue (Phast Gel ™ Blue R, GE Healthcare, Sweden).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 and 2E10.E9 Fab-PvCSPvk247 complexes were purified via Superose 6 Increase 10/300 GL chromatography (GE Healthcare) and concentrated to 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 or 2E10.E9 Fab-PvCSPvk247 complexes were purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) and diluted to 50 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... before Fab purification on a HISTrap FF column (GE Healthcare). Fab fragments were dialyzed against 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... Fab protein was purified by rProtein A Sepharose (GE Healthcare) chromatography ...
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bioRxiv - Immunology 2019Quote: ... or HiTrap KappaSelect column (GE Healthcare Life Sciences – 17545812, for Fab). The column was washed with phosphate buffered saline and eluted with 0.1 M glycine pH 2.7 ...
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bioRxiv - Biochemistry 2020Quote: ... Fab was purified by anion exchange using a Q Sepharose (GE Healthcare) column in 10 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The fluorescently labelled Fabs were passed over HiPrep desalting column (GE Healthcare) to remove the excess dye ...
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bioRxiv - Immunology 2022Quote: ... The obtained Fabs were purified with a Desalting column (GE Healthcare Life Sciences) to remove L-cysteine ...
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bioRxiv - Biochemistry 2020Quote: ... The Fab fragment was purified using ion exchange chromatography (Mono S, GE Healthcare; using a gradient of 10-500 mM NaCl in 20 mM sodium acetate ...
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bioRxiv - Biophysics 2020Quote: ... the STEAP1-Fab mixture was injected on a Superdex200 increase column (GE Healthcare) pre-equilibrated in 20 mM Tris pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Fabs were further purified on an S75 16/60 gel filtration column (GE Healthcare) using PBS (pH 7.2 ...
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bioRxiv - Immunology 2021Quote: ... Fabs and IgGs were purified from transfected cell supernatants using Ni-NTA (GE Healthcare) (for Fabs ...
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bioRxiv - Immunology 2023Quote: BAR-1 Fab was loaded onto a gel filtration Superdex 200 column (GE Healthcare) in 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The Fab and IgG proteins were purified from cell supernatants by Ni2+-NTA (GE Healthcare) and protein A affinity chromatography (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and Fab fractions were further purified by cation exchange chromatography (MonoS, GE Healthcare, Chicago, IL). His-tagged wildtype RBD (BEI NR52309) ...
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bioRxiv - Immunology 2023Quote: MIL-3 Fab was loaded onto a gel filtration Superdex 200 column 10/30 (GE Healthcare) in 10 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare). The RBD was treated with endoglycosidase H42 and Carboxypeptidase A (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2021Quote: ... Fabs were further purified by SEC using a Superdex 200 10/300 GL column (GE Healthcare Life Sciences) in 10 mM Hepes and 150 mM sodium chloride at pH 7.4.
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bioRxiv - Molecular Biology 2021Quote: ... 2F2 Fab-peptide complexes were purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare), concentrated and diluted to 10 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare), concentrated and diluted to 5 mg/mL with peptide 247-4 to immediately use in crystallization experiments.
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bioRxiv - Molecular Biology 2021Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Bioengineering 2020Quote: ... the S0_2.126/D25 Fab complex was purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2020Quote: ... The S2_1.2/Mota Fab complex was purified by size exclusion chromatography using a Superdex75 16 600 (GE Healthcare) equilibrated in 10 mM Tris pH 7.8 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the RBD-Fab complexes were purified using size-exclusion chromatography on a Superdex 200 Increase column (GE Healthcare). For both Fab 15033 and Fab 15033-7 ...
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bioRxiv - Immunology 2020Quote: ... Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure (GE Healthcare Life Sciences) column according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
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bioRxiv - Immunology 2020Quote: ... Fabs were further purified by SEC using a Superdex 200 Increase 10/300 column (GE Healthcare Life Sciences) in TBS ...
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bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Biochemistry 2020Quote: ... The conjugated Fab were then eluted from a PBS pre-equilibrated PD-mini G-25 desalting column (GE Healthcare) that removed unconjugated dye ...
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bioRxiv - Immunology 2020Quote: ... Secreted Fabs were purified from cell supernatants harvested four days post-transfection using Ni2+-NTA affinity chromatography (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... Complexes were purified from excess Fab by size exclusion chromatography using a Superose-6 10/300 column (GE Healthcare). Peak fractions corresponding to the Ab1485-BG505-8ANC195 complex were concentrated to 1.5 mg/mL in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: Antibody Fab fragments were purified further via size-exclusion chromatography using an S200 increase 10/30 column (GE Healthcare) equilibrated with 25 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... Both proteins were mixed in a molecular ratio 1:1 and the AR3C-HC84.1 Fab mixture was re-purified by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with PBS as the eluent.
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bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Microbiology 2020Quote: Full-length PbCSP was co-complexed with a molar excess of 3D11 Fab and loaded on a Superose 6 Increase 10/300 GL (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Molecular Biology 2021Quote: Full-length recombinant PvCSPvk210 or PvCSPvk247 was complexed with a molar excess of 2F2 or 2E10.E9 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: ... the flow-through containing Fab fragments was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB-Fab complexes were evaluated by Native PAGE and purified on a Superose-6 column (GE Healthcare Life Sciences) into TBS pH7.4 to remove aggregates.
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bioRxiv - Immunology 2021Quote: ... followed by purification to remove unbound Fab via size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare). Complexes were diluted to 30-50 µg/mL and immediately placed on 400-mesh Cu grids and stained with 2% (w/v ...