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Citations for GE Life Sciences :
1 - 29 of 29 citations for Ebola Virus Envelope Glycoprotein GP1 Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was buffer exchanged into 5% D2O and 95% H2O using PD10 desalting columns (GE Healthcare). CsI and NaCl were added to a final concentration of 150 mM and 1.5 M just before performing the experiments.
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution step ...
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bioRxiv - Physiology 2021Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with a HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution and filtration step ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Cell Biology 2020Quote: ... Fractions with >95% pure protein were pooled and re-purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of 0 – 300 mM NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... An equal amount of protein was boiled in Laemmli buffer for 10 min at 95°C and separated on 12.5% acrylamide gel and blotted on a nitrocellulose membrane (GE healthcare). Blocking was done with 0.5% w/v of BSA in Tris-buffered saline with 0.1% v/v Tween 20 (TBST) ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C before analysis by SDS-PAGE and transfer onto a nitrocellulose membrane (Protran, 45 µm pores, GE Healthcare). DACone adducts onto BLG were visualized by scanning the membrane with an infrared imager (Odyssey ...
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bioRxiv - Microbiology 2023Quote: ... The radiochemical purity was estimated to be > 95% based on TLC autoradiography (TLC-ARG) and HPLC (LC-20; Shimadzu) using a Superdex 200 10/300 column (GE Healthcare). 89Zr-DFO-3N39v4-Fc was administered into the tail vein (400ug ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes from the lungs of influenza virus infected mice were enriched using Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient and stained with H-2Db restricted tetramers conjugated to fluorophore R-phycoerythrin (PE ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity-purified protein was further purified (>95%) using Superdex 75 prep-grade matrix in a 16/70 C column (GE Healthcare Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Molecular Biology 2023Quote: ... was processed for amplification of complete genome of the virus by rolling circle amplification (RCA) method using TempliPhi illustra amplification kit (GE Healthcare, Piscataway, NJ). The resulting RCA product (2 μL ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...