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Citations for GE Life Sciences :
1 - 50 of 1244 citations for Dengue Virus serotype 4 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Biophysics 2019Quote: ... for 20 min at 4 0C and histidine tagged protein was recovered from the lysate using a HiTrap TALON Crude Co2+ column (GE Healthcare, Piscataway, NJ) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were loaded into a ResourceS (GE Healthcare) cartridge and eluted in a gradient of 0 to 500 mM NaCl in 50 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with lgG sepharose (GE Healthcare) at 4 °C for 2 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with glutathione sepharose beads (GE Healthcare) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysates were incubated with IgG-Sepharose (GE Healthcare) at 4 °C for 2 h and eluted by TEV protease (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... lysates were incubated with streptavidin sepharose beads (GE Healthcare) overnight while rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
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bioRxiv - Biophysics 2019Quote: ... Lysate was loaded on to HisTrap HP (GE Healthcare), His-RanQ69L was eluted in binding buffer with 500mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were loaded onto HisTrap crude column (GE Healthcare) on FPLC system (AKTA pure) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysates were incubated with IgG Sepharose (GE Healthcare), washed with buffer Y and eluted by overnight TEV cleavage.
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bioRxiv - Cell Biology 2023Quote: ... lysates were incubated with glutathione Sepharose beads (GE Healthcare) coated with the other interaction partner for 30min at 4°C on a rotary wheel ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Bioengineering 2020Quote: ... Clarified lysate loaded onto HiTrap Q HP column (GE Healthcare) and eluted through salt gradient using low-salt buffer (as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant proteins were eluted by imidazole gradient ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare), eluted with denaturing buffer supplemented with 500 mM imidazole and refolded as indicated in Table S3 using MWCO 2000 membranes (Spectra/Por) ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was purified by Histrap FF columns (GE Healthcare) with an imidazole gradient from 0 to 0.5 M ...
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bioRxiv - Cell Biology 2020Quote: ... Centrifuge-cleared lysate was applied to Ni sepharose (GE healthcare), washed with 50 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... protein lysates were incubated with glutathione Sepharose beads (GE Healthcare) in E buffer for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were incubated with prewashed glutathione sepharose (GE Healthcare) and gently mixed with rotation for 1 h ...