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Citations for GE Life Sciences :
1 - 50 of 305 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Pathology 2020Quote: ... and binding detected using streptavidin-HRP (GE Healthcare) diluted 1:4000 in 50μL EB ...
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bioRxiv - Bioengineering 2024Quote: ... Binding curves were fitted using BIAevaluation (GE Healthcare) 1:1 binding kinetics to calculate KD.
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Microbiology 2021Quote: ... Binding curves were analyzed using BIAEvaluation software (GE Healthcare) and data was fit to a 1:1 Langmuir with drifting baseline interaction model.
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bioRxiv - Biophysics 2024Quote: ... Binding data was analyzed using BIAevaluation software (GE Healthcare) and fitted to a single-site binding model.
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bioRxiv - Biophysics 2021Quote: ... ITC binding assay was performed via an ITC200 (GE Healthcare) equipment at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: γ-tubulin was purified by binding to HisTrap HP (GE Healthcare) in binding buffer (50 mM KPO4 pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... in binding buffer on an Äkta Pure FPLC (GE healthcare) with sample pump ...
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bioRxiv - Systems Biology 2022Quote: ... and its binding partner TAB1 (GE Healthcare Dharmacon: MHS6278-202760135) were PCR amplified and ligated as a fusion construct (TAK1 (1-303)-TAB1(451-end) ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on film (Kodak ...
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bioRxiv - Genetics 2023Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on G:BOX gel imaging system (Syngene ...
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bioRxiv - Microbiology 2023Quote: Column-based binding assay used heparin-agarose beads (GE Healthcare) as described (48 ...
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bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
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bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...
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bioRxiv - Biophysics 2019Quote: ... PreScission protease was removed by binding to GST-Sepharose (GE Healthcare) for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO ...
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type TANV16L as well as two mutants TANV16L K52A and R90A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Direct binding experiments were performed on a Biacore 4000 (GE Healthcare) in 10 mM HEPES (pH 7.4) ...
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bioRxiv - Genomics 2019Quote: ... ribosome protected fragments (RPF) were purified from small RNA fragments using MicroSpin S-400 HR columns (GE Life Sciences) according to the TruSeq Ribo Profile Kit (Illumina) ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2021Quote: SPR binding experiments were collected on a Biacore T200 Instrument (GE Healthcare). Anti-His6 monoclonal antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Heats of binding were measured using a MicroCal iTC200 calorimeter (GE Healthcare) with a stirring rate of 1000 rpm at 27 °C ...
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bioRxiv - Molecular Biology 2019Quote: All binding measurements were conducted using a BIAcore T100 instrument (GE Healthcare). Biotinylated RNF43 ...
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bioRxiv - Genetics 2021Quote: We performed SPR binding analysis using a Biacore T200 instrument (GE Healthcare) at room temperature in HBS-EP(+ ...
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bioRxiv - Immunology 2021Quote: SPR binding studies were performed using a Biacore T200 (GE life sciences). All studies were performed at 25°C in running buffer consisting of 10mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody binding was detected by enhanced chemiluminescence (GE Healthcare; Buckinghamshire, United Kingdom). Protein quantification was performed using a GS-900 Calibrated Densitometer (Bio-Rad Laboratories ...
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bioRxiv - Pathology 2024Quote: ... Lysates were purified through binding to Fast flow Nickel sepharose (GE Healthcare) and washed with buffer (50 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... binding curves were analyzed using BIAevaluation software (GE Healthcare, Marlborough, MA, USA) following standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplified fragments of the NP(412-500) or shorter fragments from pCAGGS-NP were cloned into pGEX-6P-2 (GE Healthcare). All sequences of the cloned or mutated DNA regions of the plasmids were validated by Sanger DNA sequencing.
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bioRxiv - Immunology 2019Quote: Binding to FcγRs was carried out using a Biacore T200 biosensor (GE Healthcare). Recombinantly expressed FcγRS (R&D systems or Sino Biologicals ...
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bioRxiv - Biochemistry 2020Quote: Measurements of heparin binding were performed in a Biacore T200 instrument (GE Healthcare) using a heparin-coated chip (Xantec) ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2021Quote: ITC binding assays were carried out on a MicroCal ITC200 calorimeter (GE Healthcare) at 25 °C in a buffer containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: All analytical SEC binding assays were conducted on an ÄKTAmicro system (GE Healthcare) using a Superose 6 Increase 3.2/300 column (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... amplified fragments were inserted into pGEX-6P1 (GE Healthcare) and the resulting plasmids were used for heterologous expression ...
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bioRxiv - Microbiology 2020Quote: ... All OtDUB fragments were cloned into pGEX6P1 (GE Healthcare) using BamHI/XhoI sites and included a sequence encoding a C-terminal Flag tag as described above ...
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bioRxiv - Neuroscience 2021Quote: ... Collagens were captured by ion exchange chromatography using a CaptoS-5mL prepackaged column (GE Healthcare) and eluted with 20 mM Tris pH 7.1 ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected by binding goat anti-rabbit IgG-HRP (NA934, GE Healthcare), goat anti-mouse IgG-HRP (NA931 ...
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bioRxiv - Biochemistry 2020Quote: Binding studies were performed at 25°C using a MicroCal iTC200 instrument (GE Healthcare). ANT(9 ...