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Citations for GE Life Sciences :
1 - 50 of 77 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Molecular Biology 2022Quote: ... and the σA subunit were expressed and purified by chromatography on HisTrap HP (GE Healthcare) Ni2+-affinity columns as described (Hu et al. ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant containing toxin was passed through Ni-affinity column (HisTrap FastFlow Crude, GE Healthcare) initially ...
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bioRxiv - Microbiology 2022Quote: ... toxins were purified through Ni2+-affinity chromatography using a HiTrap Heparin HP column (GE Healthcare). Protein capture was verified via visualization of a single band on SDS-PAGE following Coomassie blue staining ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescently labelled subunits were assessed by visualizing an SDS-PAGE gel with Typhoon FLA 9500 (GE Healthcare) with LPG filter and 100 μm pixel size ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fully formed complexes and disassembled subunits were separated on a Superose 6 10/300 column (GE Healthcare) equilibrated in SEC buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... Detection of radiolabelled mt-DNA subunits was performed using an Amersham™ Typhoon™ Biomolecular Imager (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... using buffer B (GE Healthcare Biosciences) and a flow rate of 1 mL/min ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... YrbE1A/B-MceG and YrbE1A/B-MceGK43A were further purified and analyzed by SEC (AKTA, GE Healthcare) at 4 ⁰C on a pre-packed Superdex Increase 200 10/300 GL column (Cytiva) ...
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bioRxiv - Biophysics 2020Quote: ... 25 µg/mL amphotericin B (GE Healthcare) and maintained at 26°C in non-vented ...
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bioRxiv - Biophysics 2020Quote: ... 25 µg/mL amphotericin B (GE Healthcare) and maintained at 26°C in non-vented ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Sera-Mag Speed Beads A and B (Ge Healthcare) were vortexed until the pellet was dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... ActiCHO Feed A and ActiCHO Feed B (both GE Healthcare) started on day four based on a glucose-controlled feeding regimen ...
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bioRxiv - Biochemistry 2023Quote: ... whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare). Affinity tags were cleaved by incubation with either thrombin (Merck ...
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bioRxiv - Biophysics 2019Quote: A pET-based plasmid overexpressing each subunit of RNAP (full-length α, β, ω) as well as β’-PPX-His10 (PPX; PreScission protease site, LEVLFQGP, GE Healthcare) was co-transformed with a pACYCDuet-1 plasmid containing Eco rpoZ into Eco BL21(DE3) ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare) via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Antigen B was further purified from Fc using rProteinA Sepharose (GE Healthcare), followed by deglycosylation with endoH (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 mM b-ME) and purified over a Superdex 200 column (GE Healthcare). Octamer-containing fractions were pooled according to purity and histone stoichiometry ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) and a multi-frequency linear-array transducer (L4-12T ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: B cells were purified from spleens using Ficoll® Paque Plus (GE Healthcare) gradient centrifugation and negative depletion with FITC-conjugated mAb specific for CD4 (GK1.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) was used to determine the thickness of the VL of the right leg as previously described by our laboratory (18 ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Neuroscience 2023Quote: ... and were purified b y a ffinity ch romatography (GE Healthcare, Chicago, USA). Viral titer was determined by quantitative PCR using Taq-Man technology (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: Further purification of gyrase A and gyrase B used a mono-Q (GE Healthcare) column in buffer D and a linear gradient elution from 0 to 1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Addition of nutrient feeds ActiCHO Feed A and ActiCHO Feed B (both GE Healthcare) started when glucose levels dropped below ≤ 4 g x L-1 and was continued daily thereafter ...
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bioRxiv - Immunology 2020Quote: PBMCs from Donors A and B were isolated by Ficoll-Paque (GE Healthcare, Pittsburgh, PA) gradient centrifugation and cryopreserved ...
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bioRxiv - Molecular Biology 2021Quote: ... and the respective feed media ActiCHO Feed a and ActiCHO Feed B (both GE Healthcare). Inoculation cultures were obtained by expansion of ConM and ConS lead clones from MCBs in production medium (ActiCHO P medium supplemented with 8 mM L-glutamine ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 300 mM NaCl (IMAC B buffer) and loaded onto a 5 mL HiTrap IMAC column (GE Healthcare) using an Äkta Start Chromatography system ...
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bioRxiv - Plant Biology 2021Quote: Purification of cardosin B was performed and monitored by chromatography using an AKTA system (GE Healthcare, USA). 20X concentrated protein extract (10 ml ...
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bioRxiv - Microbiology 2022Quote: ... 1mM b-ME) and further purified by Size Exclusion Chromatography (HiLoad 26/600 Superdex 200, GE Healthcare), pre-equilibrated with buffer E (20 mM HEPES pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μg of GST or GST-Aurora B or GST-ERK was added to GSH beads (GE Healthcare, USA) in binding buffer (50 mM Tris-cl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... B-mode ultrasonography of the chest was performed (18;19) using a Logiq e system (GE Healthcare, Milwaukee, WI) using R5.2.x software and a multifrequency transducer model 12L-RS at a working frequency of 10 MHz ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
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bioRxiv - Biochemistry 2020Quote: ... using a linear gradient of buffer B to increase the concentration of imidazole on an ÄKTA purifier system (GE Healthcare). The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Molecular Biology 2024Quote: Ultrasonography images of the vastus lateralis (VL) were acquired with a B-mode imaging device (LOGIQ S7 Expert, GE Healthcare) using a multi-frequency linear array transducer (3-12 MHz ...