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Citations for GE Life Sciences :
1 - 50 of 2151 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... and supernatants loaded onto 5 mL HP HIS-TRAP columns (GE Healthcare Life Sciences) equilibrated in buffer A (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4 and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of immobilised E141A H164A OmCI protein ...
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on an equilibrated 5 mL His-trap column (GE healthcare/Cytiva) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then passed through a 5 mL His-Trap FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...