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Citations for GE Life Sciences :
1 - 50 of 752 citations for CD25 Mouse Monoclonal Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Horseradish peroxidase-labeled anti-M13 monoclonal antibody (GE Healthcare) and Thermo Scientific 1-Step Ultra TMB solution were used for detection of bound phages ...
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bioRxiv - Microbiology 2024Quote: ... Secondary peroxidase labeled α-mouse (GE Healthcare), α-rabbit (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse-anti-His6 (1:1000, GE Healthcare) and monoclonal rabbit-anti-pT72-Rab8a (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Bound phages were detected using a horseradish peroxidase-labeled anti-M13 monoclonal antibody (GE Healthcare) and Thermo ScientificTM 1-StepTM Ultra TMB solution.
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were Cy3-labeled α-mouse (GE Healthcare), fluorescein-conjugated α-rat (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-labeled anti-mouse IgG antibody (GE healthcare) was used as a secondary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG horseradish peroxidase labeled secondary antibody (GE Healthcare). Proteins were detected by ECL chemiluminescent detection system (Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Molecular Biology 2019Quote: ... ZNRF3 and CD25 were coupled on a SA sensor chip (GE Healthcare) at low density ...
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bioRxiv - Genetics 2021Quote: ... The following antibodies were used: anti-GFP (7.1/13.1, mouse monoclonal IgG, secondary antibody peroxidase conjugated sheep anti-mouse IgG, NA931V, GE healthcare) or anti-Flag (M5 ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse monoclonal antibodies were purified from hybridoma supernatant using protein G columns (GE Healthcare). The specificity of CAST monoclonal antibodies was confirmed by immunohistochemistry with CAST KO mice (56) ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... we coupled 130 μg of a mouse monoclonal anti-H4K12Ac (gift from Hiroshi Kimura) to 12.5 μl rProtein A Sepharose (GE Healthcare) slurry in antibody coupling buffer (10 mM K-HEPES pH=8 ...
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bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
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bioRxiv - Cell Biology 2021Quote: ... Cy3-labeled α-goat (GE Healthcare), red TEXAS α-rabbit (Jackson Laboratories ...
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Immunology 2022Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Product #: 31433) (Pierce Antibodies) or donkey-anti-rabbit IgG (Product #: NA934V) (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Biophysics 2019Quote: ... Samples were subjected to Western blot using anti-1D4 monoclonal antibodies (University of British Columbia) and anti-mouse Horseradish peroxidase-conjugated antibodies (GE Healthcare). Protein bands were detected by enhanced chemiluminescence on X-ray film (ECL kit ...
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... labeled with Cy3 dUTPs (GE healthcare: PA53022), by means of nick translation (Abbott ...
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bioRxiv - Neuroscience 2020Quote: ... was labeled with 99mTc (GE Healthcare, Seattle, WA) using the stannous tartrate method and the 99mTc – labeled albumin (T-Alb ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes from Qiagen were labeled with Cy3 monoreactive dye (GE Healthcare) and purified by ethanol precipitation and washing with 80% ethanol until the supernatant was colorless ...
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bioRxiv - Neuroscience 2022Quote: ... was labeled with 99mTc (GE Healthcare, Piscataway, NJ). A mixture of 240 mg/ml stannous tartrate and 1 mg/ml albumin was adjusted to pH 3.0 with HCl and one millicurie of 99mTc-NaOH4 was added to this mixture and incubated for 20 min ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Molecular Biology 2020Quote: ... and probed with 5’ end-labeled oligonucleotides or PCR fragments labeled by random priming (Megaprime DNA Labeling System, GE Healthcare Life Sciences). Hybridizations were performed in UltraHyb or UltraHyb-Oligo (Ambion ...
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bioRxiv - Immunology 2022Quote: ... labeled according to the manufacturer’s recommended protocols (GE Healthcare). Arrays were immersed in a hybridization solution containing a mixture of Cy3-rabbit antihuman IgG solution diluted 1:1000 in wash buffer for 2 h at 50 rpm at 20°C ...
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bioRxiv - Immunology 2020Quote: ... a diluted secondary antibody labeled with HRP (GE healthcare) was added and incubated for 3 hours at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Labeled INIP was purified using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with INIP storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were labeled with Cy3 NHS ester (GE Healthcare) at an amine-modified internal thymine and HPLC purified on a 218TP C18 reverse-phase column (Grace/Vydac ...
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bioRxiv - Biochemistry 2023Quote: ... Labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with the storage buffers of proteins (hSSB1 ...
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bioRxiv - Biochemistry 2019Quote: ... The labeled proteins were purified using a PD10 (GE Healthcare) gel filtration column ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeled octamer was purified over a Superdex 200 (GE Healthcare) gel filtration column to remove excess dimer ...
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A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integrationbioRxiv - Molecular Biology 2024Quote: NusA was labeled with Cy5-maleimide dye (PA25031, GE Healthcare) using an 8:1 molar ratio of dye to protein in a total volume of 200 µL (∼20 µM protein ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G50-column (GE Healthcare) and extracted from a PAA gel as described above.
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bioRxiv - Biochemistry 2024Quote: ... The labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre- equilibrated with Storage buffers (hSSB1 ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled protein was purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer and dialyzed against storage buffer to remove residual free dye.
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bioRxiv - Biochemistry 2024Quote: ... the labeled protein was purified using a PD10 (GE Healthcare) gel filtration column pre- equilibrated with INIP Storage buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
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bioRxiv - Microbiology 2020Quote: ... were labeled using the Cy5 monoreactive Dye pack (PA25001, GE Healthcare) and filtered using Illustra microspin G25 columns (27532501 ...
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bioRxiv - Biochemistry 2021Quote: ... were directly labeled with Cy3 following the manufacturer’s guidelines (GE Healthcare). After 45 min incubation in the dark ...
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bioRxiv - Biochemistry 2020Quote: The N-terminus of Rad6 was labeled with Cy5 (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Labeled oligonucleotides were purified using microspin G-25 columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G-50 column (GE Healthcare), following separation in and extraction from a polyacrylamide gel as described above.
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...